Working Collagen IV Antibody From Novus Biologicals

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

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Company:

Novus Biologicals

Product Name:

Collagen IV Antibody

Catalog Number:

NB120-6586

Clone Number:

N/A

Our research focuses on neuro-vascular units in neurodegenerative diseases. The specific goal of this experiment was to evaluate vasculature in mouse models. This antibody demonstrated excellent vasculature staining in the brain.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain

Primary Incubation

1:200 at 4 degrees Celsius for overnight.

Blocking Agent

5% normal donkey serum

Secondary Incubation

Alexa fluor anti-mouse IgG at 1:500 for 1hr at room temp.

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Robust immunofluorescence signals from Collagen IV antibody labeling highlighting the basement membrane of vasculature in mouse brain tissue sections.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific.

The Bad

None.

The Bottom Line

Great Collagen IV antibody commercially available.

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