Exceptionally Good IPTG to Induce Gene Expression in Bacteria

In a previous study conducted in our laboratory, we demonstrated that the presence of multiple copies of mepM, which encodes a paralog of YgeR, compensated for the absence of the D, D-endopeptidase MepS (Singh et al., 2012). Hence, I also checked whether multiple copies of ygeR can compensate for the loss of MepS. The complete get gene was cloned into a pTrc99a vector under the control of an IPTG-inducible promoter (Ptrc::ygeR) and subsequently introduced into the ?mepS strain and checked for the rescue of the mepS knockout phenotype.