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PE anti-human HLA-A,B,C Antibody
311406
W6/32
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The goal of this experiment was to test activation and MHC I presence on cell surface in a population of activated human primary dendritic cells.
Flow cytometry
Human dendritic cells
0.1 uL Zombie Green viability stain in 100 uL PBS, incubated at room temp for 15 mins.
Human TruStain Fc block, 5 uL diluted in 95 uL FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 10 mins.
1 uL of HLA A,B,C antibody diluted in 100 uL of FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 20 mins.
Cytofix buffer, 100 uL for 20 mins at 4 degrees C.
Flow cytometry in a BD Celesta cytometer.
This product helped find HLA A,B,C (MHC I) positive cells to validate that all cells activated using our activation cocktail also presented MHC I on their cell surface.
N/A
Identifies HLA A,B,C + cells.
MHC I presentation is on a spectrum, no clear population between somewhat and very positive cells.
Ineffective and shows MHC I presentation, would buy again.