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Anti-HIF-1 alpha Antibody From Abcam With Great Signal
(1)
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Abcam
August 09, 2023
I used this antibody to check HIF1A expression in colorectal cancer cell lines, Colo205 and HT29. I used 40ug of proteins. Running was done ...
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Versatile GFP Antibody From Abcam
(1)
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Abcam
August 09, 2023
To visualize mitochondrial proteins, I use acetone methanol (1:1) fixation. However, it causes a reduction in GFP fluorescence. To overcome ...
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eBioscience™ BrdU Kit for IHC/ICC Colorimetric
(1)
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Thermo Fisher Scientific
August 09, 2023
We used the kit for labeling proliferative cells for our in vivo tumor xenografts using colorectal cancer patients' derived organoids. The ...
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Easy-to-Use RNeasy Lipid Tissue Mini Kit From Qiagen
(1)
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August 09, 2023
This kit is designed specifically for adipose tissue RNA isolation. In my case, I used this kit to help isolate RNAs from mouse mesenteric ...
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Good Antibody From BioLegend That Reliably Identifies CD86+ Cells
(1)
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BioLegend
August 09, 2023
The goal of this experiment was to identify CD86+ cells as a measure of dendritic cell activation.
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Stable Rat ADAMTS5 Elisa Kit From Krishgen Biosystems
(1)
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Krishgen Biosystems
August 07, 2023
This kit is very stable and very simple to use. The Rat ADAMTS5 Elisa kit can be used for tissue homogenates. The standard curve is very ...
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Assay Kit to Measure Proteasome Activity From Promega
(1)
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Promega
August 07, 2023
The proteasome is the main protein degradation machinery in cells. To study the effect of proteasome inhibition on the cellular proteome, I ...
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Best GAPDH Antibody From Abcam
(1)
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Abcam
August 07, 2023
I use GAPDH antibody as loading control for total cellular protein fractions or soluble protein fractions regularly under various stress ...
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Excellent Antibody for Immunofluorescence
(1)
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Cell Signaling Technology
August 07, 2023
I stained human macrophages with this rabbit anti-HMGB1 antibody to identify the HMGB1 location in the cell. It works great!
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Mitotracker Red CMXRos for Staining Mitochondria From Invitrogen
(1)
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Thermo Fisher Scientific
August 05, 2023
We used Mitotracker Red CMXRos to stain mitochondria in fixed cells and perform confocal microscopy.
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Good Protein Ladder From Invitrogen for Native Gels
(1)
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Thermo Fisher Scientific
August 05, 2023
As a reference, we use this native protein ladder for sizing estimation of proteins separated on native gels or for performing Western blots...
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Stain-Free Precast Gels From BIO-RAD for Polyacrylamide Gel Electrophoresis
(1)
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Bio-Rad
August 05, 2023
I use these gels in my research to a great extent because of the short running time, and because they are readily available (you can cut ...
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Native Gels for Separating Mitochondrial Respiratory Complexes
(1)
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Thermo Fisher Scientific
August 05, 2023
The native gels were used to separate and study the dynamic organization of mitochondrial respiratory complexes under different conditions.
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Precast Gels for Faster, Cleaner and Reproducible Results
(1)
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Thermo Fisher Scientific
August 04, 2023
To perform proteomics of cellular protein fractions, we used gel electrophoresis as a pre-separation technique.
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Staining Cell Nucleus With DAPI From Invitrogen™
(1)
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Thermo Fisher Scientific
August 04, 2023
I used DAPI to stain nucleus of the cells for visualization in confocal microscopy and observed the localization of my protein of interest.
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Specific Antibody From ThermoFisher With Some Background
(1)
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Thermo Fisher Scientific
August 04, 2023
Decent antibody and works well in the thymus, but there is some background to be expected. Does not work for flow.
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Reliable Anti-Human CD3 Antibody for Blue Laser in Flow
(1)
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BioLegend
August 04, 2023
PBMCs contain various cell types and to check the purity of T lymphocytes after the purification step, I have used an anti-human CD3-PerCP ...
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Great Staining for Human CD19 With Antibody From BioLegend
(1)
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BioLegend
August 04, 2023
Staining for human CD19 is strong and specific. Easy to separate B cells by flow cytometry. Not much background.
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Strong B220 Staining With Antibody From BioLegend
(1)
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BioLegend
August 04, 2023
The staining for B220 in mouse samples with this antibody is very strong. Easy to separate B cells by flow cytometry.
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Antibody From BD Biosciences With Very Strong IgM Staining
(1)
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August 04, 2023
Staining for IgM in human tonsils is very strong. Great antibody for studying B cells and plasma cells.
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Good Antibody From ThermoFisher, but Has Some background
(1)
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Thermo Fisher Scientific
August 04, 2023
Great antibody for IF, but there is a little background in the image. Also works for flow cytometry.
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Great GL7 Staining
(1)
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BioLegend
August 04, 2023
The staining of GL7 in GC B cells is very clear. The signal is strong and specific when analyzed by flow cytometry.
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MTT Assay for Cell Viability
(1)
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MilliporeSigma
August 04, 2023
I used the MTT assay as a simple, rapid, and reliable indicator of the cell viability of cultured cells under different treatment conditions...
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Stable and Effective CCCP From Sigma
(1)
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MilliporeSigma
August 04, 2023
CCCP is a potent uncoupler of oxidative phosphorylation and disrupts the mitochondrial membrane potential. We used CCCP as a positive ...
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LIVE/DEAD™ Cell Imaging Kit for Assessing Cell Viability
(1)
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Thermo Fisher Scientific
August 02, 2023
The assessment of cell viability in response to various drug treatments is important for my research in protein engineering. To monitor and ...
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Sanger Sequencing From Genewiz
(1)
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August 02, 2023
Sanger sequencing plays a pivotal role in my research, as it provides a robust and accurate method to confirm DNA sequences following ...
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Annexin V Apoptosis Assay for Measuring Apoptosis Using Flow Cytometry
(1)
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August 02, 2023
My research interest revolves around studying apoptosis in response to various treatments, and I accomplish this by utilizing the Annexin V ...
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Good Service for Oligo Synthesis From Elim Biopharm
(1)
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Elim Biopharmaceuticals, Inc
August 02, 2023
In my intensive protein engineering work, PCR amplification of genes is a crucial step to obtain specific DNA fragments for further ...
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Gene Block Synthesis by Twist Bioscience
(1)
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Twist Bioscience
August 02, 2023
My primary research interest lies in the captivating realm of protein engineering, where I delve into the ingenious utilization of gene ...
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Measuring Na+ Concentration Using ING-2 AM From Ion Biosciences
(1)
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July 31, 2023
I am interested in measuring sodium ion levels in cells because sodium plays a crucial role in various cellular processes, including ...
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Staining Protein Gels Using GelCode Blue Stain Reagent From Thermo Scientific
(1)
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Thermo Fisher Scientific
July 31, 2023
I work extensively with proteins, conducting protein purification experiments to analyze and study protein expression. For protein ...
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Great GFP Antibody for Western Blot and Immunofluorescence From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
July 31, 2023
I use the GFP antibody to detect the expression level of GFP-tagged proteins in my experiments. By using the GFP tag, I can easily visualize...
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Precast Gradient Protein Gels From Invitrogen
(1)
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Thermo Fisher Scientific
July 31, 2023
In my research, I work extensively with proteins and encounter a diverse range of protein sizes. To effectively analyze and separate these ...
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GTP-γ-S to Measure G-Protein Coupling Affinity of GPCRs
(1)
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Roche
July 31, 2023
I use GTP-?-S to measure the G-protein coupling affinity of serotonin 1A receptors because it allows me to assess the receptor's activity ...
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Odyssey® DLx Infrared Imaging System from LI-COR Biosciences
(1)
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LICORbio
July 30, 2023
Quantitative Western blotting is a critical technique often required in my research, and for this purpose, I rely on the Odyssey® DLx ...
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8-Hydroxy-DPAT to Measure Specific Binding to the Serotonin 1A Receptor
(1)
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July 30, 2023
As a biophysicist focused on the serotonin-1A receptor during my Ph.D., I find measuring ligand binding with radiolabeled agonist 8-Hydroxy-...
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NEPA21 Electro-Kinetic Transfection Systems for the Electroporation of Mammalian Cells
(1)
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July 30, 2023
In my research, I aim to study protein localization and interactions by transiently transfecting cells using electroporation. ...
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Best Anti-α-Tubulin Antibody to Use in Western Blotting From MilliporeSigma
(1)
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MilliporeSigma
July 30, 2023
In my research, I aim to unravel the NFAT5 signaling mechanism by employing various protein biochemistry techniques, including protein ...
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Highly Sensitive Pico PLUS Chemiluminescent Substrate
(1)
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July 30, 2023
As a researcher, working with proteins expressed at extremely low levels has been quite challenging when conducting Western blot experiments...
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X-tremeGENE™ 9 DNA Transfection Reagent for Transfecting Mammalian Cells
(1)
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Roche
July 30, 2023
In my research, I aim to study protein localization and interactions by transiently transfecting cells using transfection. Transfection is a...
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cAMP Gi HTRF Kit for Assaying Gi-coupled GPCR Signaling From Cisbio
(1)
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July 30, 2023
I am interested in studying the activity and function of serotonin 1A receptors, which are coupled to Gi signaling pathways. To investigate ...
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Amplex™ Red Cholesterol Assay Kit From Invitrogen
(1)
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Thermo Fisher Scientific
July 29, 2023
I am interested in measuring membrane cholesterol upon statin treatment from mammalian cells. I used Amplex™ Red Cholesterol Assay Kit for ...
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Reliable BCA Protein Assay Kit
(1)
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Thermo Fisher Scientific
July 29, 2023
This kit was used to measure protein content from cell lysates. A standard curve is generated with known concentrations of BSA and unknown ...
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Measuring Membrane Dipole Potential With Di-8-ANEPPS
(1)
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Thermo Fisher Scientific
July 29, 2023
I investigated the use of Di-8-ANEPPS to measure the membrane dipole potential. The fluorescent dye Di-8-ANEPPS is utilized as a sensitive ...
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TLC Silica Gel 60 Plates for Separation of Lipids
(1)
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MilliporeSigma, Merck
July 29, 2023
TLC Silica gel 60 plates are utilized for separating lipids from cell membrane extracts, enabling the identification and quantification of ...
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Excellent Staining With MHC-II Antibody From BD Biosciences
(1)
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July 29, 2023
Great staining of MHC-II on mouse immune cells from intestinal single cell suspensions. Very bright staining.
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cOmplete™ His-Tag Purification Resin for purifying His-tagged protein
(1)
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Roche
July 29, 2023
I work with in-vitro protein phase separation assays which require high-quality purified proteins. The cOmplete™ His-Tag Purification Resin ...
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Dual-Luciferase Reporter Assay System to Detect Firefly and Renilla Luciferase Activities
(1)
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Promega
July 29, 2023
The Dual-Luciferase® Reporter Assay System has proven to be an invaluable tool in my research for measuring target gene transcription. By ...
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Highly Sensitive ECL Western Blotting Substrate From ThermoFisher
(1)
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Thermo Fisher Scientific
July 29, 2023
As a protein biochemist studying protein levels and protein-protein interactions, Western blotting is an indispensable technique for my ...
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Amicon® Ultra-15 Centrifugal Filters for Concentrating Proteins From Millipore
(1)
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MilliporeSigma
July 29, 2023
Amicon® Ultra-15 Centrifugal Filters are essential for concentrating proteins in biological research and biotechnological applications. ...
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