BioLegend
Brilliant Violet 421™ anti-human CD40 Antibody
334332
5C3
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The goal of this project was to identify activated cells in a CD11c+ and CD14+ (dendritic cell) primary human cell population after stimulation with an activation-inducing cytokine cocktail.
Flow cytometry
Human dendritic cells
0.1 uL Zombie Green viability stain in 100uL PBS, incubated at room temp for 15 mins.
Human TruStain Fc block, 5 uL diluted in 95 uL FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 10 mins
1uL of CD40 antibody diluted in 100 uL of FACS buffer (5% FBS in DPBS), incubated at 4 degrees C for 20 mins.
Cytofix buffer, 100 uL for 20 mins at 4 degrees C.
Flow cytometry in a BD Celesta cytometer.
This product helped identify activated cells (CD40+ in combination with CD80/86) dendritic cells, to verify that our maturation and activation cocktails produce mature and activated dendritic cells. We were able to identify that almost all of our cells were CD40+.
N/A
Good identifier of CD40+ cells.
Very bright, requires voltage adjustment.
Very bright antibody that identifies CD40+ cells.