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Exceptionally Good IPTG to Induce Gene Expression in Bacteria
(1)
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MilliporeSigma
August 23, 2023
In a previous study conducted in our laboratory, we demonstrated that the presence of multiple copies of mepM, which encodes a paralog of ...
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Great Vortex Mixer From Labnet
(1)
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Corning Life Sciences
August 23, 2023
I study cell walls in the context of bacterial growth and the associated enzymes. For in vitro biochemical assays, I extract cell walls as ...
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BUV496 Anti-Human CD16 Flow Cytometry Antibody From BD Biosciences
(1)
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August 22, 2023
I wanted to stain the expression of CD16 on the surface of peripheral NK cells and monocytes. So, I stained human PBMCs in serial dilutions ...
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BUV805 Anti-Human CD56 Flow Cytometry Antibody From BD Biosciences
(1)
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August 22, 2023
I wanted to stain the expression of CD56 on the surface of peripheral NK cells. Therefore, I stained human PBMCs in serial dilutions from 1:...
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BV510 Anti-Human CD14 Flow Cytometry Antibody From BioLegend
(1)
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BioLegend
August 22, 2023
I wanted to stain the expression of CD14 on the surface of peripheral classical monocytes for a dump channel of sorting. So, I stained human...
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BV605 Anti-Human HLA-DR Flow Cytometry Antibody From BioLegend
(1)
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BioLegend
August 22, 2023
I wanted to stain the expression of HLA-DR on the surface of peripheral myeloid cells. So, I stained human PBMCs in serial dilutions from 1:...
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BUV615 Anti-Human CD56 From BD Biosciences Works Well
(1)
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August 21, 2023
I used it for phenotyping human PBMC by flow cytometry to study inflammatory disease. 5 lase Cytek Aurora was used, and it works great.
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Best Novus Biologicals Antibody Against LXR Alpha
(1)
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Novus Biologicals, a Bio-Techne Brand
August 21, 2023
The aim of the study was to test the effect of a growth factor treatment on the human primary cell line on LXR alpha protein levels. For ...
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Antibody for Complex I Subunit NDUFS1 From Abcam
(1)
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Abcam
August 21, 2023
NDUFS1 is one of the subunits of mitochondrial respiratory complex I. We used it as a representative subunit to see distribution across ...
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VDAC Antibody for Mitochondrial Loading Control From Abcam
(1)
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Abcam
August 21, 2023
To study mitochondrial proteins and their activity, I isolated mitochondria from cells. To check the purity of mitochondria and the ...
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Antibody by Abcam With Good Staining But Some Background is Present
(1)
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Abcam
August 21, 2023
This antibody was used to mark nervous tissue to assess the response of the rabbit anal sphincter to injury and treatment.
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Working Collagen IV Antibody From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
August 21, 2023
Our research focuses on neuro-vascular units in neurodegenerative diseases. The specific goal of this experiment was to evaluate vasculature...
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ROS Detection Assay Kit From Abcam
(1)
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Abcam
August 21, 2023
In my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on ...
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anti-human CD4 BUV661 works well
(1)
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August 21, 2023
I used it for phenotyping human PBMC by flow cytometry to study inflammatory disease. 5 lase Cytek Aurora was used, and it works great.
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Good Abcam Antibody for the Immunodetection of Parkin in Human Cells
(1)
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Abcam
August 21, 2023
Human cells were treated with growth factors for different periods. The cells were lysed in RIPA buffer, and proteins were extracted. The ...
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BUV563 Mouse Anti-Human CD69 From BD Biosciences Works Great
(1)
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August 21, 2023
I used it for phenotyping human PBMC by flow cytometry to study inflammatory disease. 5 lase Cytek Aurora was used and it works very well.
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Great Specific Staining of Skeletal Muscle With Antibody From Abcam
(1)
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Abcam
August 21, 2023
This antibody was used to mark skeletal muscle to assess the response of the rabbit anal sphincter to injury and treatment.
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Great Antibody From Novus Biologicals to Detect FABP4 in Human Cells
(1)
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Novus Biologicals, a Bio-Techne Brand
August 20, 2023
In the present experiment, the human primary cells were exposed to a specific treatment for different time intervals, and the tissue samples...
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Fantastic Monoclonal Anti-6X His Tag Antibody From Millipore
(1)
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MilliporeSigma
August 20, 2023
To study the functionality of a bacterial cell wall hydrolase (MepK), I have generated catalytically inactive variants of MepK in pTrc99a ...
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Ni-NTA Matrix With High Binding Capacity From Qiagen
(1)
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August 20, 2023
We study the role of cell wall hydrolases in the context of bacterial cell wall synthesis. To assess the biochemical activity of these cell ...
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Great CD11c+ Marker From BioLegend
(1)
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BioLegend
August 20, 2023
The goal of this project was to mature CD14+ PBMCs (monocytes) into dendritic cells, then analyze activation in the presence of activation-...
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Lyophilized Micrococcus Lysodeikticus Cells From Millipore for Zymogram Analysis.
(1)
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MilliporeSigma
August 20, 2023
I have biochemically characterized a cell wall hydrolase, MepK. To assess its in vitro activity, I initially performed a Zymogram analysis ...
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Cephalexin, an Antimicrobial Agent by Sigma
(1)
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MilliporeSigma
August 20, 2023
I identified a putative cell wall hydrolase through a genetic screen. After creating a knockout of this gene, I aimed to determine its ...
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An Exceptionally Responsive Dye From Invitrogen for Visualizing DNA Within Agarose Gels
(1)
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Thermo Fisher Scientific
August 20, 2023
I study genes related to bacterial cell wall biology. While cloning these genes, I use this dye daily to make agarose gels to confirm my PCR...
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Antibody from BioLegend Easily Identifies CD40+ Cells
(1)
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BioLegend
August 20, 2023
The goal of this project was to identify activated cells in a CD11c+ and CD14+ (dendritic cell) primary human cell population after ...
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Good Marker From BioLegend for MHC I+ Cells
(1)
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BioLegend
August 18, 2023
The goal of this experiment was to test activation and MHC I presence on cell surfaces in a population of activated human primary dendritic ...
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Excellent Experience With Primordium
(1)
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August 18, 2023
Cell wall hydrolases are integral to bacterial physiology. In my research, I have constructed many plasmids for complementing genes in ...
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Good PerCP/Vio700 CD8 Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 18, 2023
I wanted to stain the expression of CD8 on the surface of peripheral T cells, therefore I stained human PBMCs in serial dilutions from 1:25,...
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Great MBP Antibody From Proteintech
(1)
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Proteintech Group Inc
August 18, 2023
The goal of this experiment was to access myelin status in disease progression in neurodegenerative diseases such as multiple sclerosis and ...
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Seamless Experience With Azenta
(1)
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August 18, 2023
I need to make knockouts in bacteria by swapping the gene of interest with an antibiotic resistance marker. I have used Azenta for verifying...
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Assay Kit From Promega Identifies Viable/ATP Containing Cells With Some Margin of Error
(1)
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Promega
August 18, 2023
The goal of this project was to quantify intracellular ATP as a measure of cell viability and immunogenic cell death after treatment with ...
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Great Marker for Lymphatic Vessels From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
August 18, 2023
Our research focuses on myeloid cell pathology in neurodegenerative diseases. The specific goal of this experiment was to evaluate ...
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Straightforward and Easy-to-Follow IFN Gamma ELISA Kit From Thermo Fisher
(1)
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Thermo Fisher Scientific
August 18, 2023
I'm usually hesitant to order uncoated ELISA kits because of the presumed inconvenience and variability from lot to lot but I had to because...
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PE Anti-Human CD127 Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of CD127 on the surface of peripheral NK cells. Therefore, I stained human PBMCs in serial dilutions from 1...
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APC Anti-Human NKG2C Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of NKG2C on the surface of peripheral adaptive NK cells. Therefore, I stained human PBMCs in serial ...
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Excellent AF700 Anti-Human CD7 Antibody From BioLegend
(1)
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BioLegend
August 17, 2023
I wanted to stain the expression of CD7 on the surface of peripheral ILCs for sorting. Therefore I stained human PBMCs in serial dilutions ...
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PE/Vio770 Anti-Human CD1c Flow Cytometry Antibody From Miltenyi Biotec
(1)
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Miltenyi Biotec
August 17, 2023
I wanted to stain the expression of CD1c on the surface of peripheral cDC2s. Therefore, I stained human PBMCs in serial dilutions from 1:25,...
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BV510 CD69 Flow Cytometry Antibody From BioLegend
(1)
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BioLegend
August 17, 2023
To test this antibody, I stained PBMCs for 30 minutes at room temperature in the dark at serial dilutions of 1:25, 1:50, 1:100, 1:200, and 1...
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Anti-Human PD-1 BUV737 From BD Biosciences Works Well
(1)
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August 16, 2023
I used it for phenotyping human PBMC by flow cytometry to study inflammatory disease. 5 lase Cytek Aurora was used and it works great.
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Good Beta-Tubulin Antibody From Abcam
(1)
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Abcam
August 16, 2023
I use beta-tubulin antibody as a loading control for protein gels as it does change under various stress conditions.
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Great Marker for Lymphatic Vessels From Abcam
(1)
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Abcam
August 16, 2023
The specific goal of experiments using this antibody was to assess alterations in the lymphatics in disease status. This antibody worked ...
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Must Have Imaging System for Cell Culture Labs From Invitrogen
(1)
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Thermo Fisher Scientific
August 16, 2023
Transient transfections, cell staining, and stable cell line preparation are frequent and common experiments that I perform while using cell...
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A Great Blood Vessel Endothelium Marker From Novus Biologicals
(1)
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Novus Biologicals, a Bio-Techne Brand
August 16, 2023
Our research focuses on understanding the role played by glia-vascular interaction in neurodegenerative diseases. The specific goal of this ...
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Fluorescent Dye From Thermo Fisher for Mitochondria Membrane Potential
(1)
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Thermo Fisher Scientific
August 16, 2023
TMRM is a cell-permeant, cationic, red fluorescent dye that is readily sequestered by active mitochondria and can be detected by either ...
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Good Beta-Actin Antibody From Abcam
(1)
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Abcam
August 16, 2023
I use actin antibody as a loading control for my Western blots. It especially works great for insoluble protein fractions.
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Convenient DNA Gel Extraction Kit From Qiagen
(1)
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QIAGEN - Sample to Insight
August 16, 2023
This kit is very useful for extraction of DNA from agarose gels especially if you have non-specific PCR-amplified DNA bands.
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Best Transfection Reagent From Thermo Fisher
(1)
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Thermo Fisher Scientific
August 16, 2023
I frequently perform cell culture transfections of my protein of interest tagged with fluorescent proteins for either stable cell line ...
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Anti-Mouse IFN-γ Antibody for Flow Cytometric Quantification
(1)
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BioLegend
August 15, 2023
I study tumor immune microenvironment in preclinical primary and metastatic models of colorectal cancer. My research involves extensive ...
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Activation Cocktail for Cytokine Quantification by Flow Cytometry
(1)
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BioLegend
August 15, 2023
My research area includes immune phenotyping of primary or metastatic tumors. I often do ex vivo stimulation of T cells sorted from the ...
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Good and Cheap MYC Tag Antibody From Proteintech
(1)
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Proteintech Group Inc
August 15, 2023
The antibody works great for detecting myc-tagged proteins in Western blot. It gives a good signal even in higher dilutions.
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