Abcam
Anti-ARL2 Antibody
ab109742
N/A
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We were looking for an antibody to test the effect of a growth factor on the ARL2 protein level in mammalian cells. Our antibody search ended with the Abcam, and it was inspiring to see the antibody specificity which was indicated in the flyer. Thus, to complete our goal, the log phase of growth, arrested cells were treated with specific growth factors for a different period. From these cells, the protein extract was prepared and resolved in 12 % SDS-PAGE. The resolved protein was western transferred on the nitrocellulose membrane and probed for ARL2 using Abcam Ab.
Western blot
Mammalian whole-cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degrees celsius.
5% skimmed milk made in 1x TBST.
Goat anti-mouse IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT.
None
Enhanced chemiluminescent detection in X-Ray films.
Abcam antibody has detected a band of ARL2 with another high molecular weight non-specific band in the mammalian protein sample.
A specific band of ARL2 was observed.
Other nonspecific bands were detected.
Good Abcam antibody for Immunodetection of ARL2 in mammalian cells.