Miltenyi Biotec
CD27 Antibody, anti-human, PE
130-113-630
M-T271
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B-cells in health and disease. Here, we aimed at staining plasma cells (CD27+CD38+) isolated from human bone marrow.
Flow cytometry
Human bone marrow from a total hip arthroplasty
Surface staining with CD27 PE (1/50), 20 min at 4 C.
FC block
N/A
Flow cytometer
On the figure, on the left there is the expression of CD27 vs CD38 gated on CD14-CD3-dapi- (live B lineage cells). A very nice population of CD38hiCD27hi PCs can be observed. On the right there is an histogram of CD27 expression.
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