Abcam
Anti-Cullin 2/CUL-2 antibody
ab133180
N/A
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Browse Similar Products: Anti-Cullin 2 Western Blot Antibody Products
The study aimed to test the effect of an on-target siRNA on Cullin 2/CUL-2 protein levels in human cells. The transfected cells were transfected with on-target siRNA, and after incubation for 36 hrs, the cultured cells were synchronized and treated with a growth factor. The cells were lysed, protein extracts were made, and resolved in 10% SDS-PAGE. The resolved proteins were immunoblotted with Cullin 2/CUL-2 antibody to detect their levels. The selection of antibodies was based on information provided in the antibody flyer.
Western blot
Human cell protein extract
Dilution (1:1000) in 5% skimmed milk prepared in 1x TBST, overnight incubation at 4 degrees celsius.
5% skimmed milk made in 1x TBST.
Goat anti-rabbit IgG HRP Ab, dilution (1:4000) in 5% skimmed milk made in 1x TBST, 1hr at RT.
None
Enhanced chemiluminescent detection in X-Ray films.
Abcam Cullin 2/CUL-2 antibody detected a very light band of Cullin 2/CUL-2 protein. However, this antibody has detected several other nonspecific epitopes too.
Gives a band of Cullin 2/CUL-2
Very light and faint band was detected.
Average Cullin 2/CUL-2 antibody.