Abcam
Anti-Apolipoprotein A I antibody [EP1368Y]
ab52945
N/A
Info: Supplier Page View Product Specs
Browse Similar Products: Anti-Apolipoprotein A-I Antibody Products
Growth-synchronized human smooth muscle cells were subjected to treatment with growth factor treatment. The cells were further tested to measure the effect of growth factor on Apolipoprotein A I levels by immunoblotting techniques. After resolving proteins from each treatment, the protein-transferred nitrocellulose membrane was probed with Apolipoprotein A I antibody. Abcam antibody was selected because of the information indicated in the company flyer.
Western blot
Human cell protein extract
Dilution (1:1000) in 5% BSA prepared in 1x TBST, overnight incubation at 4 degrees celsius.
5% skimmed milk prepared in 1x TBST.
Goat anti-rabbit IgG HRP Ab, dilution (1:5000) in 5% skimmed milk prepared in 1x TBST, 1hr at RT.
None
Enhanced chemiluminescent detection in X-Ray films.
The indicated antibody has detected a bright specific band of Apolipoprotein A I, and this shows the specificity of this antibody was high for its on-target epitope.
A specific single band of Apolipoprotein A I protein.
None.
Excellent antibody.