Good Beta-III Tubulin Antibody From Novus Biologicals

September 06, 2023

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

beta-III Tubulin Antibody

Catalog Number:

NB100-1612

Clone Number:

N/A

The specific goal of the project is to assess retinal ganglion cell (RGC) damage in optic nerve damage. This antibody worked great as a marker for RGC soma, axons, and dendrites in mice.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse retina

Primary Incubation

1:500 for 3 days at room temp

Blocking Agent

5% normal donkey serum

Secondary Incubation

Alexa fluor anti-chicken IgG at 1:500 for overnight at room temp

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

Beta-III Tubulin is a well-known retinal ganglion cell marker. This antibody worked great and showed high specificity and robust signals when used for mouse flatmounted retina.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Robust signal.

The Bad

None.

The Bottom Line

Highly recommended Beta-III Tubulin antibody for immunofluorescence.

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