Excellent RBPMS Antibody from PhosphoSolutions

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

PhosphoSolutions

Product Name:

Anti-RBPMS Antibody

Catalog Number:

1830-RBPMS

Clone Number:

N/A

Our lab research focuses on neurodegeneration in glaucoma. The specific goal of the project is to assess retinal ganglion cell damage in the context of optic nerve damage. This antibody worked great as a specific marker for mouse retinal ganglion cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse retina

Primary Incubation

1:500 for 3 days at room temp.

Blocking Agent

5% normal donkey serum.

Secondary Incubation

Alexa fluor anti-rabbit IgG at 1:500 for overnight at room temp.

Tertiary Incubation

N/A

Detection

Fluorescence

Results Summary

This antibody worked well as a tool to assess retinal ganglion cell survival after optic nerve injury. RBPMS is well known as a retinal ganglion cell specific marker, allowing quantification of RGC loss. This antibody showed little background and high specificity when used for mouse flat mounted retina.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very robust signal.

The Bad

None.

The Bottom Line

A great RBPMS antibody with reproducible results.

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