Invitrogen
IL-33R (ST2) Monoclonal Antibody (hIL33Rcap), PerCP-eFluor™ 710, eBioscience™
46-9338-42
N/A
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I used it for phenotyping human PBMC by flow cytometry to study immune memory in inflammatory disease. 5 lase Cytek Aurora was used. It was very difficult to detect IL33R positive population, but I assume it is because of rare population not the antibody itself.
Flow cytometry
Human PBMC
1:50
Fc block
I tittered the antibody and ended up using 1:50 dilution with 5% FBS 2mM EDTA in PBS. Incubation for 30min RT works well and shows positive population although it is very rare.
Looks like it is working.
None.
The positive population is very rare, but the antibody still works.