BioLegend
Brilliant Violet 605™ anti-mouse CD3 Antibody
100237
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The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD3 Antibody from BioLegend specifically because CD3 is expressed in T cells and can be found in lymph nodes. Using this product I was able to differentiate lymphocyte subsets.
Flow Cytometry
Lymph node
1:200 30' in ice
FcBlock
NA
Fluorescence
Target expression is very low but fluorochrome pretty bright. Best if spillover into the channel is minimized.
None
Important reagent to have
Target is dim
Useful to have