Best Bioruptor for ChIP Assays

May 14, 2015

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RAJIV GANDHI CENTRE FOR BIOTECHNOLOGY
MYCOBACTERIUM RESEARCH GROUP
SENIOR RESEARCH FELLOW

Overall

Performance

Ease-of-Optimization

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Company:

Diagenode

Product Name:

Bioruptor Standard

Catalog Number:

B01010002 (UCD 200 TO)

We use the Bioruptor for our Chromatin immuno-precipitation assays. It is essential for us to optimize the size of DNA to fit our requirements before antibody pull-down. This product allows us to do that.

Experimental Design and Results Summary

Tips

Make sure the the sonicator tub is at 4 degrees celsius. If the machine is hot then stop for a while and then restart. Never give more than 10 cycles during a single stretch. Make sure the sample is maintained at lower temperature.

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Summary

The Good

User friendly.

The Bad

No automatic temperature control. Becomes hot if we don't monitor the temperature every time.

The Bottom Line

Good machine that regularly works on ChIP experiments. Gives control over DNA chopping for ChIP experiments which is very important.

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