Abcam anti-human Fut4 ab131406 for western blot

Molecular Biology
Princeton
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Fut4

Catalog Number:

ab131406

Info: View Product View Product Specs

Browse Similar Products: FUT4 Antibodies

Ab131406 was tested at 1/250 on human cells over-expressing human Fut4 by traditional Western blot. No specific staining was detected.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cancer cells

Primary Incubation

1/250 at 4C o/n

Blocking Agent

5% milk

Secondary Incubation

1:10000 Licor 800CW

Tertiary Incubation

NA

Detection

LICOR scanner

Results Summary

No specific detection of the overexpressed protein was found, nor were endogenous levels detected. No other optimization steps were attempted. Expression was verified by an alternate antibody.

Additional Notes

None

Related Categories

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Summary

The Good

None

The Bad

Non-working

The Bottom Line

Non-working for human Fut4 by Western blot

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