Dim Fluorochrome CD21/CD35

CSB
MGH
postdoc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BD Pharmingen™ PerCP-Cy™5.5 Rat Anti-Mouse CD21/CD35

Catalog Number:

562797

Browse Similar Products: CD21/CD35 Antibodies

The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD21/CD35 Antibody from BD specifically because CD21/CD35 is expressed in B cells and can be found in lymph nodes. Using this product I was able to differentiate B cell subsets.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph node

Primary Incubation

1:300 30' in ice

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fluorescence

Results Summary

Fluorochrome is dim and bleeds into several other channels (PC7, Bv711, APC) but still is useful if the multicolor panel is well planned.

Additional Notes

None

Image Gallery

Summary

The Good

Important reagent to have

The Bad

Not very bright

The Bottom Line

Good to go

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