Beta Actin Antibody in Western-Blot

Vascular Oncology
DKFZ
PhD Student

Overall

Quality of Results

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Company:

Novus Biologicals

Product Name:

Beta Actin Antibody

Catalog Number:

NB600-501

Info: View Product View Product Specs

Browse Similar Products: Beta-Actin Antibodies

The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. In order to understand their functional roles, we downregulate the candidate genes in different cell lines and verify it with Western Blot by using specific antibodies. Even with a very high dilution this antibody is able to detect the specific Beta Actin in Western-Blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Caco-2 cells

Primary Incubation

Dilution: 1/15000, time: 1 hour, room temperature

Blocking Agent

5% milk powder solution in 1xTBST

Secondary Incubation

Anti mouse HRP (Dilution: 1/5000, time: 30 min, room temperature)

Tertiary Incubation

NA

Detection

ECL detection

Results Summary

Even with a very high dilution this antibody is able to detect the specific Beta Actin in Western-Blot.

Additional Notes

None

Related Categories

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Summary

The Good

Specific, strong signal at low concentration of the primary antibody

The Bad

Nothing bad

The Bottom Line

Perfect antibody for the detection of Beta Actin in Western-Blot.

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