Cell Signaling Technology
PTEN Antibody (138G6)
9559
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The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. In order to understand their functional roles, we downregulate the candidate genes in different cell lines and verify it with Western Blot by using specific antibodies. By using this antibody the specific band for PTEN is detected without any problem.
Western Blot
T84, MCF7 whole cell lysates
Dilution: 1/1000, time: 1 hour, room temperature
5% milk powder solution in 1xTBST
Anti mouse HRP (Dilution: 1/5000, time: 30 min, room temperature )
NA
ECL detection
By using this antibody the specific band for PTEN is detected without any problem.
None
Specific, strong signal at low concentration of the primary antibody
Nothing bad
This antibody would be a good choice for specific detection of PTEN in Western Blot.