The Good
Easy use, economical for this application. No cross-over with the other fluorescence channel.
The Bad
FITC signal may be weak, hence fluorochromes with higher intensity and less photobleaching, if available, may be used.
The Bottom Line
This antibody can be used to detect classically activated macrophages. In applications where mixed cell populations are the norm, such as in the peritoneal exudate, use of other macrophage-specific markers (such as F4/80) should be combined with this antibody.