TRYPSIN 0.25% 100ML 15050065 from Thermo Fisher Scientific

Product Specs
ItemTRYPSIN 0.25% 100ML
CompanyThermo Fisher Scientific
Price $12.17Supplier Page View Company Product Page
Catalog Number15050065
Conjugate/TagUnconjugated
Format0.25%
1X
Liquid
Molecule NameTrypsin
Size100mL
ApplicationsCELL CULTURE
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) A protocol for untargeted metabolomics using anion-exchange chromatography and high-resolution mass spectrometrySTAR ProtocAugust 19, 2026Marius Jouan, Sylvain Henry, Andrei Turtoi,Thermo Scientific,61870036 "Penicillin-streptomycin (5,000 U/mL)",Thermo Scientific,15070063 0.25% Trypsin EDTA,Thermo Scientific,15050065 FBS,Thermo Scientific,A3160501 PBS,Thermo Scientific,14190144 ,, Experimental models: Cell lines,Experimental models

(2) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelPLoS PathogAugust 4, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O'Shea, Ari Bel, et al.48–72 hours and the cells were passaged at 90% confluency in using 0.25% Trypsin-EDTA (catalog number 15050065, Thermo Fisher Scientific). The rAAV vector production and purification followed a previously established

(3) Baicalein Attenuates High Glucose and Sodium Palmitate-Induced Ferroptosis in Cardiomyocytes via the Nrf2/SLC7A11/GPX4 Signaling PathwayInt J Mol SciJuly 18, 2026Huan Wang, Yuhan Xu, Lei Wang, Wanting Meng, Yanwu Xu, Haidong GuoPB180327), digested with 0.25% trypsin (Gibco, Thermo Fisher Scientific, Grand Island, NY, USA; Cat. No. 15050065), and subsequently passaged or seeded for further experiments. 4.2. Cell Viability Assay To determine

(4) Sequential release of N-butylphthalide via multifunctional hydrogel for rapid neuroprotection and sustained neural repair after traumatic brain injuryMater Today BioJuly 6, 2026Changbin Hu, Ning Li, Yuanyuan Ran, Hao Fu, Lei Zhou, Fanglei Li, Chenye Qiao, Chuhan Liu, Guiqin Tian, Guoping Guan, et al.followed by flow cytometry. Cells were collected by trypsinization using 0.25% trypsin (EDTA-free; Gibco, 15050065) for 2 min at 37°C, neutralized with DMEM containing 10% FBS (Gibco, 11965118), and centrifuged at 1000

(5) Intercellular chemerin-cmklr1 couples epicardial mechanosensing to fibroblasts in pressure overloadNature CommunicationsJune 19, 2026Guowei Zeng, Xinjie Zhang, Longming Huang, Kai Luo, Xiaoyang Zhang, Bo Chen, Cong Li, Haoyu Li, Jinghao Zheng, Bozhong Shi, et al.neonatal mice, minced, and digested overnight at 4 °C in HBSS containing 0.01 mg/mL trypsin (Gibco, #15050065). The following day, the predigested tissue fragments were transferred to a solution containing 1500

(6) TRMT10C promotes mitochondrial fission through transferrin receptor m1A methylation modification and aggravates the progression of atrial fibrillationJ Cell Commun SignalJune 10, 2026Nijina Li, Hao Zhangtissues were sheared and digested in 37°C for 7‐to‐8 min. The digestion enzymes included trypsin (0.25%, 15050065, Thermo Fisher Scientific) and collagenase type II (0.67%, 17101015, Thermo Fisher Scientific). After

(7) Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in MelanomaAdvanced ScienceMay 26, 2026Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, et al.dilution [69]. Briefly, cells were dissociated with 0.25% EDTA‐free trypsin (Thermo Fisher Scientific, 15050065; 3 min), diluted to 1000 cells/mL in complete DMEM, and seeded into five 96‐well plates by mixing 240

(8) Calcitriol is involved in maintaining primordial follicle reserve through the inhibition of the PI3K/Akt signaling pathwayJournal of Ovarian ResearchMay 21, 2026Shuang Liu, Ye Chen, Hongwei Wei, Huiyu Liu, Weiyong Wang, Yanli Sun, Luchun Zhang, Sihui He, Xiaodan Zhang, Jingjie Li, et al.isolated from 4 dpp mice, and then were digested with 0.05% trypsin (Thermo Scientific, Waltham, MA, USA. 15050065) at 37 °C for 10 min. The digestion was stopped by adding 10% fetal bovine serum (FBS, Thermo Scientific.

(9) Conditional knockout of Dkk3 drives Lgr5+ progenitor reprogramming into hair cells in the mouse cochleaTheranosticsMay 18, 2026Hairong Xiao, Xinlin Wang, Zixuan Ye, Xin Tan, Ying Ma, Xiangyu Ma, Wei Tong, Luying Zhang, Yanqin Lin, Xujun Tang, et al.ice-cold PBS (1×, Gibco, 10010023) and enzymatically dissociated using 150 μL 0.25% trypsin (Gibco, 15050065) at 37 °C for 10 min. Mechanical dissociation was completed by pipetting up to 300 μL trypsin inhibitor

(10) Acidic-responsive nano liposomes potentiate in situ dual immunotherapy on lung cancer by codelivering antagonistic peptide and agonistInt J Pharm XMay 6, 2026Yinshan Lin, Lu Liang, Le Wang, Minyan Wei, Qingjun Yang, Xiaoling Guan, Qiuyun Liu, Youguang Pan, Jianfen Su, Lingmin Zhangwith PBS (10010023, Gibco,) and digested with trypsin without Ethylenediaminetetraacetic acid (EDTA, 15050065, Gibco,). Cells were centrifuge at 2000 rpm for 5 min and stained by Annexin V-FITC/PI (C1062S, Beyotime)

(11) CRP Deficiency Rescues Periodontitis‐Induced Hippocampal Neurogenesis Impairment by Suppressing OPC‐Derived BMP4 Signaling in RatsAdvanced ScienceMay 1, 2026Lingjie Li, Ping Deng, Siyu Hou, Xianbo Xia, Wei Zhao, Xingyu Zhu, Yang Zhang, Chao Wang, Ling Xu, Jinlin Songfragments and digested for 15 min at 37°C with a digestion solution containing 0.25% trypsin (Gibco, 15050065), DNase I (MCE, HY‐108882), and Hanks’ balanced salt solution (HBSS, Beyotime, C0218). The digestion

(12) Orderly mitosis shapes interphase genome architectureeLifeApril 21, 2026Krishnendu Guin, Adib Keikhosravi, Raj Chari, Gianluca Pegoraro, Tom Mistelitook 5–7 days to achieve confluence. The cells were then harvested by using trypsin (Gibco, Cat. No. 15050065). A portion of the cells was frozen to generate a polyclonal stock, and the remaining cells were taken

(13) Orderly mitosis shapes interphase genome architectureElifeApril 21, 2026Krishnendu Guin, Adib Keikhosravi, Raj Chari, Gianluca Pegoraro, Tom Mistelitook 5–7 days to achieve confluence. The cells were then harvested by using trypsin (Gibco, Cat. No. 15050065). A portion of the cells was frozen to generate a polyclonal stock, and the remaining cells were taken

(14) Enhanced cell contact and planarian tissue extract increase DNA replication and viability for neoblast cultivationiScienceApril 17, 2026Wenya Zhang, Ling Yang, Nan Li, Kai LeiT6365 Mitomycin C,Sigma,Cat# M5353 Poly-D-lysine,BD Biosciences,Cat# 354210 0.25% trypsin,ThermoFisher,Cat# 15050065 36.5-38% Formaldehyde solution,Sigma,Cat# F8775 2%Bis-acrylamide Solution,Leagene,Cat# PE0147 40%Acrylamide

(15) FOXE1 promotes the progression of pulp inflammation by activating PANoptosis in dental pulp cellsiScienceApril 17, 2026Gu F, Li R, Huang D, Peng L, Huan T, Ye K, Bian Z, Yin W.6-well plates and treated as indicated. After stimulation, cells were harvested with 0.25% trypsin (#15050065, Gibco), centrifuged and labelled with PI and FITC-conjugated annexin V using a commercial apoptosis

(16) Saikosaponin D and paeoniflorin improve the HCC immune microenvironment via CLCF1/PD-L1 mediated crosstalk between CAFs and tumor cellsScientific ReportsApril 13, 2026Kun Zhou, Wei Yuan, Fan ZhangPasadena, CA, USA). Detection of apoptosis First, the cells were collected by tryptic digestive solution (15050065, Gibco). Then, the cells were washed with PBS and suspended in a binding buffer. They were then treated

(17) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.72 hours and the cells were passaged at 90% confluency in using 0.25% Trypsin-EDTA (catalog number 15050065, Thermo Fisher Scientific). The rAAV vector production and purification followed a previously established

(18) Monosodium Glutamate Inhibits Pseudomonas aeruginosa-Induced Acute Lung Injury by Targeting the Type III Secretion Systems and Modulating Host ImmunityMicroorganismsMarch 23, 2026Jing Xu, Weiwei Wang, Yaxin Zhou, Hongxing Zhang, Zixuan Shang, Zhijin Zhang, Bing Li, Yubin Bai, Jiyu ZhangRPMI 1640 medium (Cat#: 11875101), penicillin/streptomycin (Cat#: 15140122), 0.25% trypsin-EDTA solution (Cat#: 15050065), fetal bovine serum (Cat#: FBP-C520), and LiveBLAzer™ FRET-B/G Loading Kit (containing CCF4-AM) were

(19) ZNF219 inhibits porcine epidemic diarrhea virus by degrading viral S2 proteinVirologica SinicaMarch 20, 2026Wenli Zhao, Ning Kong, Daoliang Lan, Yu Zhang, Hailong Liu, Chunyun Liu, Wenzhen Qin, Xinyu Yang, Tian Liu, Wu Tong, et al.PBS (Gibco, C20012500BT), and challenged at 0.01 or 1 MOI along with trypsin (4 μg/mL; Invitrogen, 15050065). Before harvesting, the cells were washed with PBS and assessed for viral proliferation via Western blotting

(20) Protocol for identifying surface membrane proteins and their associated proteome from mouse cortical neuron cultures by in situ biotinylationSTAR ProtocMarch 20, 2026Liang Shi, Pritha Bagchi, GiaLinh N. Nguyen, Victor Faundez, Gary J. Bassell21022CV HEPES Buffer Solution 1M,Cytiva,Cat# SH30237.01 "Trypsin (0.25%), phenol red",Gibco,Cat# 15050065 Trypsin (0.25%) with EDTA,Gibco,Cat# 25200-056 Trypan Blue,Invitrogen,Cat# T10282 "Boric Acid (>99.5%,

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(1)

10/4/2023Great Trypsin From Gibco Aditi KothariI have been using it for years. I trust this brand for my media reagents. I would highly recommend it.

TRYPSIN 0.25% 100ML from Thermo Fisher Scientific

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TRYPSIN 0.25% 100ML from Thermo Fisher Scientific

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