Very Good Anti-Human CD8 antibody

May 14, 2015

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

APC-H7 Mouse anti-Human CD8

Catalog Number:

560179

Browse Similar Products: CD3 Flow Cytometry Antibodies

Human PBMCs were isolated from healthy individuals and the cells were stained with anti human CD4 and CD8 antibody. The results showed clear CD4 and CD8 positive populations, which confirms the specificity of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 minutes

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry on APC/Cy7 Channel

Results Summary

The results show a clear CD8 positive and negative population, which confirms the specificity of the antibody.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Great specificity

The Bad

Titration is required

The Bottom Line

Very good alternate for CD8 APC/Cy7 antibody

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