DLC8 Antibody in Western Blot

Vascular Oncology
DKFZ
PhD Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

GeneTex

Product Name:

DLC8 antibody [EP1660Y], N-term

Catalog Number:

GTX61597

Info: View Product View Product Specs

Browse Similar Products: DLC8 Antibodies

The main purpose of our work is to identify new functional roles of candidate genes in the cellular network. In order to understand their functional roles, we downregulate the candidate genes in different cell lines and verify it with Western Blot by using specific antibodies. Using this antibody the specific band for DLC18 is detected in Western Blot without any problem.

Experimental Design and Results Summary

Applications

Western Blot

Sample

T84 whole cell lysate

Primary Incubation

Dilution: 1/15000, time: 1 hour, room temperature

Blocking Agent

5% milk powder solution in 1xTBST

Secondary Incubation

Anti rabbit HRP (Dilution: 1/5000, time: 30 min, room temperature )

Tertiary Incubation

NA

Detection

ECL detection

Results Summary

Using this antibody the specific band for DLC18 is detected in Western Blot without any problem.

Additional Notes

None

Related Categories

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Summary

The Good

Specific, strong signal at low concentration of the primary antibody

The Bad

Nothing bad

The Bottom Line

Very good antibody for the specific detection of DLC8 in Western Blot

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