Novus Biologicals
LIF R alpha Antibody (MM0455-9B23) [Alexa Fluor ® 700] 100 Tests
NBP2-11774AF700
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Hek cells were transfected with LIFR gene. To enrich the population, transfected cells were analyzed first by flow cytometry and then sorted by FACS. The re-culturing of FACS sorted cells was successful.
Flow Cytometry
HEK-LIFR cells
90 min, 5 ul per sample (100 ul), room temperature, DPBS with Ca and MG
1% FBS
NA
Abs didn't stain negative cells. The highest 10% LIFR expressing cells were successfully sorted and re-cultured for further studies.
None
Abs worked
We were completely satisfied with this product
From our experiment it can be concluded that these Abs are suitable for FACS