Quality Kit for Plasmid Isolation

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Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

HiSpeed Plasmid Midi Kit

Catalog Number:

12643

I use kit to isolate plasmid DNA from bacteria and get good yield. The DNA is good to use with downstream cloning applications.

Experimental Design and Results Summary

Application

Plasmid Isolation

Starting Material

None

Protocol Overview

Grow 200 ml LB + bacteria. Spin down bacteria to a pellet. Follow midiprep protocol and isolate DNA.

Tips

Follow protocol

Results Summary

The kit yields a couple hundred nanograms per microliter concentrations of plasmid DNA with good spec. readings

Additional Notes

None

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Summary

The Good

Good yield and easy to use kit

The Bad

Price of kit is a little pricey

The Bottom Line

This is a good kit even if it is slightly expensive.

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