Cell Signaling Technology
NF-κB p65 (D14E12) XP® Rabbit mAb
8242
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We grow murine bone marrow derived macrophages and treat them with lipopolysaccharide to determine response of NFkB signaling with a time course.
Western Blot
Bone Marrow Macrophages
1:1000 (recommended) O/N 4C, 5% Milk TBST
5% Milk TBST
Anti-Rabbit 2e HRP conjugated
NA
Perkin Elmer ECL
The antibody works great and is specific for total p-p65, as the blot indicates. It's a great tool to differentiate between p-p65 and p65.
None
Specific detection, reliable
Highly recommend!