Propidium Iodide for Flow Cytometer Use

Propidium iodide is a common dead cell staining dye, which can be purchased from many vendors. I got this from fisher because they usually provide nice dyes. Overall, it worked OK to get a readable result with flow cytometer FL2 or FL3. One good quality about this product is that it does not have debris after dissolving in water, as in my experience PI tends to have debris after dissolving in water and filter-sterilization is required. One drawback is that the staining of cells is not very strong, compared to other products I have used before.