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CD93 PE Cy7
136506
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Our lab is concerned with studying the transcriptome and signaling changes that occur during transitional B-cell development. This is a major checkpoint in peripheral tolerance where autoreactive B-cell clones are purged. Typically, splenocytes from C57BL/6 and various mutant strains are used.
Flow Cytometry
C57BL/6 splenocytes
15-20min in FACS buffer
Fc block 5min prior
NA
Flow cytometry
CD93 PE Cy7 was used to gate on transitional B-cells. This antibody showed great separation for an antibody where separation is typically hard to achieve.
1:200 final dilution for 1 million cells
CD93 PE Cy7 works well to study transitional B-cells in both the spleen and BM
None
I would recommend this antibody for studying the transitional B-cell phenotype.