CD44 BV605

Microbiology and Immunology
University of Miami
Post-doctorall fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD44 BV605

Catalog Number:

563058

I am studying splenic B-cell development and tolerance mechanisms. The goals of my experiment were to phenotype spleens from various genotypes. CD44 BV605 was used to assess B-cell activation using this surface marker. It provided a wide range spectrum of staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

20min ice

Blocking Agent

Fc Block 5min

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

CD44 BV605 stain works well at 1:200 at 1 million cells in 200uL volume.

Additional Notes

None

Related Categories

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Summary

The Good

CD44 BV605 is able to separate activated B-cells from non-activated.

The Bad

None

The Bottom Line

This antibody can be used to distinguish activated from non-activated B cells

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