Time Course Effect of Chemotactic Agent on pAkt in HASMS

August 26, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

Phospho-Akt (Ser473)

Catalog Number:

9271

Info: View Product View Product Specs

Browse Similar Products: p-Akt Antibodies

Protein kinase B also known as Akt, belongs to a serine/threonine family of kinases. It is involved in various process such as stimulation of glucose and uptake by insulin, cell survival, transcription and cell migration. Here we checked the time course effect of pAkt in HASMS by a chemotactic agent.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Cell line

Primary Incubation

1:1000 in 5% BSA prepared in 1X TBST

Blocking Agent

5% BSA from Sigma prepared in 1X TBST

Secondary Incubation

1:4000 in 5% skim milk prepared in 1X TBST

Tertiary Incubation

NA

Detection

GAR

Results Summary

There is no change in the phosphorylation of AKT in HASMS by chemotactic agent.

Additional Notes

None

Related Categories

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Summary

The Good

There is no/less background, the antibody is very efficient and the signal is very good.

The Bad

None

The Bottom Line

This antibody is highly efficient and its detects the appropriate band with good intensity. This antibody is highly recommended for performing western blot.

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