Quantitative ELISA - Murine IgG2a Standard

Harvard Medical School
Vaccine & Virology Research
Research Fellow

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Company:

Sigma-Aldrich

Product Name:

IgG2a, Kappa murine myeloma

Catalog Number:

M9144

To quantitatively measure murine IgG2a in your sample, a standard is required within the quantitative ELISA. This IgG2a kappa, from a murine myeloma, is provided at known concentration to allow use as a standard, and works reproducibly.

Experimental Design and Results Summary

Application

Quantitative ELISA

Starting Material

Monoclonal UPC, purified immunoglobulin, buffered aqueous solution

Protocol Overview

This monoclonal standard is dissolved in PBS/1%BSA/0.05% Tween-20, at a starting concentration of 1000 ng/ml, before 5 fold dilutions.

Tips

Confirm protein concentration by OD 280nm reading

Results Summary

Provides a standard which is reproducible between ELISA plates and between experiments.

Additional Notes

None

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Summary

The Good

Availability, price, efficacy

The Bad

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The Bottom Line

A required standard in quantitatively measuring murine IgG2a within a sample by ELISA.

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