CD69 PE Flow Antibody for Detection of Activated B-Cells

August 27, 2015

Microbiology and Immunology
University of Miami
Post-doctorall fellow

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Company:

BD Biosciences

Product Name:

CD69 PE

Catalog Number:

553237

My project involves studying transcriptomic changes that occur during transitional B-cell development in the spleen. Experiments typically involve C57BL/6 mice as well as various mutant strains.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

C57BL/6 splenocytes

Primary Incubation

15-20min in FACS buffer

Blocking Agent

Fc block 5min prior

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

C57BL/6 mice were injected with Listeria Monocytogenes for 18-48 hours. Splenocytes were stained with various surface markers including activation marker CD69. Increased CD69 was observed after 18 hours but disappeared after 48 hours. Both immature (CD24hi) and mature (CD24lo) B-cells were positive for CD69 after 18 hours.

Additional Notes

None

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Summary

The Good

This antibody worked in detecting CD69 after exogenous stimulation

The Bad

The range of detection was limited but detectable

The Bottom Line

This is a good activation marker to use in conjunction with other known B-cell activation markers such as CD86

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