Good Antibody for Staining Mouse/Rat Foxp3

August 28, 2015

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse/Rat Foxp3 APC

Catalog Number:

17-5773-80

Info: View Product View Product Specs

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I am working on autoimmunity and I primarily use this antibody for the characterization of CD4+CD25+Foxp3+ Treg cells via flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse CD4+ T cells

Primary Incubation

1:500 in 100uL FACS Buffer 4 degrees for 1h

Blocking Agent

FACS buffer containing 2% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Becton Coulter CyAn

Results Summary

I use this antibody to study Foxp3 expression in CD4+CD25+ T-cells. I got good results with this antibody every time.

Additional Notes

None

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Summary

The Good

Ease of optimization

The Bad

Quite expensive

The Bottom Line

Worth buying this antibody

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