Phospho-Akt Antibody Used for Western Blot

pathology
upmc
research associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Akt

Catalog Number:

13038p

I do cloning for animal experiments to use as liver cancer models and thus use a variety of molecular biology reagents including antibodies, plasmids, and RNA kits. Phospho-AKT is a very good antibody for Western blot and IF staining.

Experimental Design and Results Summary

Applications

Western blot and IF staining

Sample

Protein lysis or frozen block section

Primary Incubation

Just follow the normal protocol

Blocking Agent

Just follow the normal protocol

Secondary Incubation

Just follow the normal protocol

Tertiary Incubation

Just follow the normal protocol

Detection

ECL

Results Summary

Rabbit mAb recognizes endogenous levels of Akt1 protein only when phosphorylated at Thr308. This antibody also recognizes endogenous levels of Akt2 protein when phosphorylated at Thr309 or Akt3 protein when phosphorylated at Thr305

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

None

The Bottom Line

None

Share your experience with other scientists. Write a Review! »

Join the discussion