Sigma-Aldrich
IgA, Kappa murine myeloma
M1421
It is of interest to measure antigen specific or total IgA concentrations in murine serum or mucosal secretions. To quantify the amount of IgA, a standard is required from which a quantitative ELISA is read. This IgA standard reviewed here provides accurate, precise and reproducible IgA standard curves in a murine ELISA protocol.
Quantitative ELISA
Monoclonal TEPC 15, purified immunoglobulin, buffered aqueous solution
The IgA, Kappa murine meloma is diluted to a concentration of 1000 ng/ml in PBS/1%BSA/0.05%Tween20, before serially dilutions depending upon the required standard curve.
Confirm protein concentration by OD 280nm reading
This purified IgA provides consistent, precise standard curves between quantitative ELISA plates and between experiments, from which the specific or total IgA concentration within the sample can be confidently determined.
None
Availability, efficacy
A good purified murine IgA to be used as a standard in quantitative ELISAs.