Time Course Effect of Chemotactic Agent on CCR2 in HASMS

August 25, 2015

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Novus Biologicals

Product Name:

C-C chemokine receptor type 2 (CCR2)

Catalog Number:

NB110-55674

Browse Similar Products: CCR2 Antibodies

CCR2 is a cell surface receptor which couples to G proteins. It further interacts with the chemokine ligands and its receptors by increasing the intracellular calcium ion level. It exsits as two isoforms and has been found to be involved in monocyte and macrophage differentiation. Here we checked the time course effect of chemotactic agents on CCR2 expression.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell line

Primary Incubation

1:500 in 5% skim Milk prepared in 1X TBST

Blocking Agent

5% skim Milk (blotting grade blocker) in 1X TBST

Secondary Incubation

1:4000 in 5% skim milk prepared in 1X TBST

Tertiary Incubation

NA

Detection

GAR

Results Summary

There is no change in the expression level of CCR2 by a chemotatctic agent.

Additional Notes

None

Related Categories

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Summary

The Good

There is no/less background and the antibody detects the appropriate band.

The Bad

It gives some non-specific bands and it takes some time to get the signal.

The Bottom Line

This antibody is very good for western blotting and there is not much background with this antibody.

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