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Date Published
Overpriced But Necessary
(1)
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October 07, 2015
We use streptavidin-coated beads to capture various biotinylated targets. The magnetic beads are collected using neodymium magnet stands for...
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Cell Mask Orange Plasma Membrane Stain fluorescent microscope
(1)
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Thermo Fisher Scientific
October 06, 2015
I am comparing the size of the endoplasmic reticulum to the size of the whole cell in a variety of treatment conditions. The ER was ...
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Very Useful Cell Counting Piece of Equipment
(1)
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Thermo Fisher Scientific
October 06, 2015
Pros: This is a great device. It is capable of counting a broad spectrum of cell sizes and densities. It counts cells within clumps pretty ...
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Anti-HA (16B12) Biolegend
(1)
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BioLegend
October 06, 2015
U2OS cells transfected with HA-SceI were lysed in sample buffer and proteins were separated in 4-20% gradient SDS-PAGE. Ha-SceI expression ...
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Identifies alpha Tubulin in Western Blots
(1)
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Santa Cruz Biotechnology, Inc.
October 06, 2015
We carry out subcellular fractionation and verify the isolated fractions by western blot analysis. We use a tubulin as a cytosolic marker.
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Electrophoretic Mobility Shift Assay (EMSA)
(1)
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October 06, 2015
EMSA is performed to study the protein-DNA interactions which exists as a complex. Due to the protein-DNA complex it will migrate slower ...
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Western Blot Analysis of GLUT 1
(1)
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Santa Cruz Biotechnology, Inc.
October 06, 2015
Testing GLUT 1 antibody as a marker for plasma membrane.
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Review of Anti-Ki67 eFluor450
(1)
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October 06, 2015
We wanted to examine if our TCR transgenic CD4 T cells were proliferating in response to an autoantigen straight out of an animal without ...
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Merck Amicon columns to concentrate RNA
(1)
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MilliporeSigma
October 05, 2015
I have used the Merck Millipore columns to concentrate Total RNA. I wanted to maximise RNA recovery by using a number of sample aliquots for...
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Anti-Foxp3 Stains the Nucleus of Tregs in the Mouse Intestine
(1)
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October 05, 2015
We wanted to examine the localization of Foxp3+ T-cells in healthy vs diseased mice, so we turned to this antibody for immunofluorescence ...
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Anti-Human CD127 Antibody for FACS Analysis
(1)
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October 05, 2015
I use this antibody for the characterization of human Treg cells based on CD+CD25+Cd127low criteria. Cd127 is an additional marker for ...
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Cell Violet Staining Kit for Measuring Cell Proliferation
(1)
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Thermo Fisher Scientific
October 05, 2015
I use this staining kit to measure T-cell proliferation induced by our stimuli. Since I have FITC conjugated antibodies in my staining ...
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Review of CD4+ T Cell Isolation Kit via Negative Selection
(1)
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October 05, 2015
We routinely perform adoptive transfer of CD4 T cells into B6.Thy1.1 recipients and analyze their proliferation in response to antigen. The...
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Western Blot Analysis of MFN1
(1)
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Novus Biologicals, a Bio-Techne Brand
October 05, 2015
In this experiment, we were studying pro-fusion proteins of mitochondria in cancer cells.
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Efficient RNase Decontamination Wipes
(1)
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October 05, 2015
Cells were grown and treated with growth factor to test its effect on mRNA expression. RNA was extracted with conventional TriZol method. ...
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Paraformaldehyde from EMS Fixes Cells Well
(1)
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Electron Microscopy Sciences
October 02, 2015
Our lab utilizes paraformaldehyde fixation for immunohistochemistry/immunofluorescence and for fixation of cells for flow cytometry. We ...
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High Quality Restriction Enzyme for Cloning
(1)
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New England Biolabs
October 02, 2015
I used this BglII restriction enzyme along with EcoRV for the cloning of my gene of interest into a plasmid vector. Subsequently, I also ...
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RNA Extraction Kit
(1)
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Omega Bio-Tek, Inc.
October 02, 2015
I use this kit in the lab to extract RNA from cells. The RNA Integrity is quite high and it is an easy kit to use.
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Fantastic Antibody to Stain a Small Population
(1)
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BioLegend
October 02, 2015
We study the distribution of gamma delta cells in response to minicircle DNA injections. With such a small subset, a good flurochrome ...
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Decent Antibody
(1)
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BD Biosciences
October 02, 2015
The Alexa700 conjugated anti-mouse CD44 antibody is a decent antibody for this super dim fluorochrome.
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SIV Mac gp140 Protein for Use in ELISAs
(1)
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Immune Technology
October 02, 2015
For characterising antibody responses to SIV mac gp140 following vaccination or natural infection, ELISAs can be carried out to quantify ...
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High Fidelity Restriction Enzyme for Cloning
(1)
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New England Biolabs
October 01, 2015
I used this EcoRI-HF restriction enzyme for one way (Single enzyme digestion) cloning of my gene of interest in to plasmid vector. ...
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DTT
(1)
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October 01, 2015
This DTT powder is used to make us 1M DTT solutions for use in reducting samples for western blot. The DTT solutions made from this powder ...
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CCR2 FACS Antibody Using Primary Monocytes
(1)
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BioLegend
October 01, 2015
One day prior the experiment, isolate monocytes using Ficoll gradient centrifugation. Afterwards, use magnetic bead separation to extract ...
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Good and Easy to Use Flow Antibody
(1)
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BioLegend
October 01, 2015
This antibody is designed for flow cytometry and the application notes state the suggested concentration clearly. It is easy to try and fast...
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Sigma REDExtract-N-Amp is Great for Quick Mouse Tail Genotyping
(1)
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October 01, 2015
We utilize the REDExtract kit to quickly obtain DNA from mouse tails for genotyping. We looked into using this product because it was much ...
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Anti-HA from Sigma Is Good Quality but Expensive
(1)
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October 01, 2015
The HA tag is short, so a clean and specific antibody is essential. Sigma's anti-HA is generally a good antibody for western blot and ...
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FGF-Basic
(1)
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Thermo Fisher Scientific
October 01, 2015
For our experiment, we needed to derive Glioma stem cells from a U87 cell line. This product was added as part of the culture for the GBM-...
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AF700 Anti Mouse CD3 in Flow Cytometry for Mouse Tissue
(1)
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BioLegend
September 30, 2015
The aim of this experiment was to detect cytokines in mouse T cells. This antibody is good to distinguish the T cell population.
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8 Sample Magnetic Stand
(1)
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September 30, 2015
This is a simple yet effective magnetic stand that has been used in conjunction magnetic beads for immunoprecipitations.
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K9 Immunophenotyping Using CD14 BV650 for Cell Surface Staining
(1)
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BioLegend
September 30, 2015
To perform Imunophenotyping on dog samples for diagnosis of lymphoma. BV650 CD14 was selected to stain for monocytic and few granulocytic ...
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Anti-CD69 Stains Activated Mouse Intestinal T-Cells
(1)
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September 30, 2015
Here, we wanted to compare the proportion of activated T-cells in a healthy vs diseased mouse. To do this, we chose to use CD69 as a marker ...
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Works for Stripping Near-Infrared Western Blots
(1)
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LICORbio
September 30, 2015
We use western blots and in-cell westerns to analyze changes in protein expression or modification in tissues and cells. After trying ...
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Great DNA Marker
(1)
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Promega
September 30, 2015
I used promega 1 kb ladder to check the product size of my PCR product. I preferred this product because it has premixed dye thus it's ready...
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Nice DNA Ladder
(1)
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New England Biolabs
September 30, 2015
Bring a molecular biologist, I often use Gel electrophoresis to compare the size of amplicon. Here, I am showing the amplication of ...
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DNeasy Blood Tissue Kit
(1)
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QIAGEN - Sample to Insight
September 30, 2015
We are assessing Aryl hydrocarbon receptor activation in bone marrow derived dendritic cells. Precursor cells are isolated by density ...
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Immunocytochemistry of Caveolin1
(1)
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Novus Biologicals, a Bio-Techne Brand
September 30, 2015
We used caveolin-1 as a marker protein to label lipid rafts in the plasma membrane.
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Excellent DNA Polymerase for PCR Amplification of Larger Fragments
(1)
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New England Biolabs
September 30, 2015
I used this polymerase to amplify a 4.5kb assembly PCR product for cloning. Earlier, we had issues with other Taq polymerases. After using ...
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Anti-Ifng Stains Ifng-Producing T-cells
(1)
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September 29, 2015
Here, we wanted to compare the proportion of Ifng-producing cells in healthy vs diseased mice. To do this, we used this anti-Ifng antibody ...
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Excellent Mouse IL-6 ELISA Detection Kit
(1)
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BioLegend
September 29, 2015
We aim to examine the pro-inflammatory status of our mutant mice. Determination of IL-6 protein level from serum collected from peripheral ...
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Bad Jagged 2 antibody
(1)
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Abcam
September 29, 2015
The aim of the study was to test the role of growth factor on Jagged 2 expression in a mammalian cell line. Cells were treated with growth ...
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Flow Cytometry Antibody Anti-Human CD11c
(1)
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BioLegend
September 29, 2015
I used this antibody to characterize human bone and monocyte derived dendritic cells. I was looking for a cheaper antibody and I got this ...
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Anti-Ly6c Stains Mouse Intestinal Monocytes
(1)
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BD Biosciences
September 29, 2015
Here, we wanted to compare the intestinal Ly6c+ monocyte population in healthy vs diseased mice. To do this, we utilized this product to ...
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Vortexer
(1)
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Scientific Industries, Inc.
September 29, 2015
This is used to vortex solution to either get things in solution or to assure a homogeneous mixture. The vortex is compact so it does not ...
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1,1,3,3-tetraethoxypropane
(1)
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September 29, 2015
TBARS (Thiobarbituric acid reactive substances) levels are measured in the plasma by using the standard curve of malondialdehyde prepared by...
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Phospo STAT5 Antibody for Western Blot and Flow Cytometry
(1)
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Cell Signaling Technology
September 28, 2015
I use this antibody to measure STAT5 activation in mouse CD4+ T cells upon treatment with our stimuli. I was looking for a antibody which I ...
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Great Mounting Media
(1)
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Thermo Fisher Scientific
September 28, 2015
We study human sinus disease, and in this particular study we were looking at tight junction histology with immunofluorescence. The slow ...
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Anti-Human FOXP3 Antibody for FACS Analysis
(1)
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September 28, 2015
I use this antibody to characterize human Treg cells based on CD4+CD25+ CD127low FOXP3 criteria. I also use this antibody to measure ...
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Anti-IL-17A stains IL-17A-producing T-cells
(1)
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September 28, 2015
Here, we wanted to examine the percentage of IL-17A+ T-cells in healthy vs diseased mice. To do this, we utilized this antibody to stain T-...
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PGE2 Measurement in Saliva Samples
(1)
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Cayman Chemical
September 28, 2015
We measured levels of PGE2 secretion in saliva samples collected from smokers and nonsmokers. We found comparable levels of PGE2 between ...
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