Novus Biologicals
Caveolin-1
NB100-615
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Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We used caveolin-1 as a marker protein to label lipid rafts in the plasma membrane.
Immunofluorescence
Cancer cells
1:200 to 1:400 in 2.5% goat serum, overnight at 4 C
2.5% goat serum
1:300, one hour at room temperature
NA
Olympus confocal microscope
We were able to stain caveolin-1 on human cancer cells to locate lipid rafts.
None
Very good antibody for ICC. This antibody also works very well for western blot.
Good antibody for tracking lipid rafts on a cell.