Anti-HA from Sigma Is Good Quality but Expensive

Molecular Micro
Wash U
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Sigma

Product Name:

Sigma anti-HA primary antibody from rabbit

Catalog Number:

H6908

Browse Similar Products: HA Antibodies

The HA tag is short, so a clean and specific antibody is essential. Sigma's anti-HA is generally a good antibody for western blot and immunofluorescent microscopy, but has defects such as it cannot be diluted too much, and is expensive.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HA-overexpression plasmid in bacteria cells

Primary Incubation

1:2000, 1 hour, RT in 5% milk/TBST

Blocking Agent

5% milk/TBST

Secondary Incubation

2% milk/TBST

Tertiary Incubation

NA

Detection

Thermo Scientific ECL

Results Summary

I get a clean band with this antibody, which is very satisfactory. I tried some other anti-HAs from other companies, it did not give me such a good and clean blot.

Additional Notes

None

Image Gallery

Summary

The Good

Clean antibody, easy to use with any secondary antibody

The Bad

Dilution limit to 1:2000, expensive

The Bottom Line

Makes research possible

Share your experience with other scientists. Write a Review! »

Join the discussion