Western Blot Analysis of GLUT 1

Biology
University of Houston
Graduate student

Overall

Quality of Results

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Glut 1

Catalog Number:

sc-7903

Info: View Product View Product Specs

Browse Similar Products: Glut1 Antibodies

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. Testing GLUT 1 antibody as a marker for plasma membrane.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:1000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

The glut antibody gives bands and can be used as a plasma membrane marker.

Additional Notes

None

Related Categories

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Summary

The Good

Works well for western blot

The Bad

Sometimes a few nonspecific bands appear in the western blot.

The Bottom Line

This antibody can be used as a plasma membrane marker.

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