Thermo Fisher Scientific
FGF-Basic (AA 10-155) Recombinant Human Protein
PHG0024
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For our experiment, we needed to derive Glioma stem cells from a U87 cell line. This product was added as part of the culture for the GBM-Cancer stem cells.
To derive & culture glioblastoma cancer stem cells from a glioblastoma cell line.
U87 cell line.
For 10ml- DMEM/F12 -9ml,540ul, N2 supplement(100x)-0.1ml, B27 supplement-(50x)-0.2ml, bFGF(10UG/ml)-20ul(final 20ng/ml), EGF(10UG/ml)- 20ul(final 20ng/ml), penstrep solution-0.1ml(1x), 250mg/ml BSA stock solution-20ul (final conc 0.5mg/ml). Filter it with a syringe filter.
Make the required amount of media, do not freeze thaw the bFGF.
The glioma cancer stem cells were derived from u87, confirmed with the relevant marker (CD133) in western blot.
None
Easy to use.
Nothing bad with the product.
Suggested to use for culturing the glioma cancer stem cells.
Product Name: FGF-Basic (AA 10-155) Recombinant Human ProteinSupplier Page