Fig 1: Deglycosylation of native scabies mite peritrophin 1 and binding to the human complement factor MBL.Scabies mite homogenates were incubated for 24 hours (upper panel) or 3 days (bottom panel) in either buffer alone (lane 1), or with PNGase F (lane 2), four O-glycosidases (lane 3), or PNGase F and four O-glycosidases (lane 4) at 37°C. The preparations were separated by SDS-PAGE in comparison with a sample of fresh mite homogenate (lane 5). Western blots were probed for SsPTP1 using anti-CBD1 sera (A), or for MBL using an anti-MBL antibody after incubation of the blot with 50% normal human serum (B) or with buffer without serum (C). In blot (C) an additional lane was loaded with 1% normal human serum in PBS (lane S). Arrows point to a single band corresponding to the 32kDa subunits of human MBL. The experiment was repeated three times.