eBioscience
Anti-Human CD127 PE-Cyanine7
25-1278
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I use this antibody for the characterization of human Treg cells based on CD+CD25+Cd127 low criteria. Cd127 is an additional marker for characterizing human FOXP3 Treg cells and can be used for FACS analysis.
Flow Cytometry
Human PBMCs and T cells.
1:500 in 100uL FACS Buffer 4 degrees for 1h
0.5% BSA FACS buffer
NA
Becton Coulter CyAn
I use this antibody for the staining of human Treg cells and to sort them out from effector T cell population. This stain works well and gives a distinct population to facilitate sorting.
None
Ease of optimization and quality of results
Nothing except cost
Worth buying this antibody.