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Date Published
Bcl-xL Expression in Jurkat Cells
(1)
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Cell Signaling Technology
October 16, 2015
The aim of the research was to determine the levels of Bcl-xL in Jurkat cells after treatment with various cytokines. Jurkat cells were ...
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Great Secondary Antibody for Visualizing Rat Primary Antibodies in the AlexaFluor 488 Channel
(1)
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Thermo Fisher Scientific
October 16, 2015
When I stain tissue sections with a rat-derived primary antibody, I utilize this product to visualize the rat primary antibody stain in the ...
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B Cell Marker
(1)
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BioLegend
October 16, 2015
The aim of this experiment was to detect the cytokines in mouse B cells. This antibody works well to distinguish the B cell population.
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Ethidium Bromide Stains DNA Very Clearly
(1)
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Thermo Fisher Scientific
October 15, 2015
Our lab runs a lot of genotyping PCRs and, to visualize the PCR products, we often use ethidium bromide.
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The Best FACS Antibody for Phospho-STAT3 (Tyr705)
(1)
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Cell Signaling Technology
October 15, 2015
Our lab is interested in T cell signaling in the context of autoimmunity and tolerance induction. In this project I'm analyzing the effect ...
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Electrophoresis machine
(1)
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Bio-Rad
October 15, 2015
The power supply is good as you can save multiple programs and consistently works. You can also choose to set the voltage or amps constant. ...
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Good Antibody for Detecting GFP
(1)
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BioLegend
October 15, 2015
The aim of this experiment was to study the phosphorylation of Rb-analog, Whi5 in budding yeast after release from G1 arrest caused by ...
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Canine Immunophenotyping
(1)
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BioLegend
October 15, 2015
This product was used to perform immunophenotyping on dog samples for diagnosis of lymphoma. This isotope control was selected to serve as a...
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Excellent CD69 Antibody
(1)
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October 15, 2015
This antibody was to used to identify recently activated T cells and it worked very well.
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Extremely Strong Antibody Against V5 Epitope
(1)
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Thermo Fisher Scientific
October 15, 2015
The aim of this experiment was to study the phosphorylation of Rb-aanalog, Whi5 in budding yeast after release from G1 arrest caused by ...
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Premixed and Ready-To-Use Solution for DNA Synthesis
(1)
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Promega
October 14, 2015
GoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for ...
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Great UV Vis Spectrophotometer for a Tight Budget
(1)
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VWR International
October 14, 2015
I needed an affordable UV Vis spec for a new lab to do basic quantification of proteins, nucleic acids, etc. Looking at various budget ...
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Best Available ATF2 Antibody for Immunofluorescence
(1)
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Cell Signaling Technology
October 14, 2015
Our aim was to identify mechanisms underlying ATF2 subcellular localization since nuclear localization is associated with poor prognosis in ...
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Good Antibody for Staining B Cells
(1)
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BioLegend
October 14, 2015
I used this antibody for staining B cells within lymphocytes. Compared to other fluorophores (BV510 etc.), this antibody showed much ...
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Excellent Antibody for Detecting Endogenous G6PDH
(1)
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October 14, 2015
The aim of this experiment was to study the expression dynamics of budding yeast G1 cyclin Cln2 after release from a G1 block caused by ...
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GFP Antibody
(1)
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Santa Cruz Biotechnology, Inc.
October 14, 2015
I transfected 293T cells with a vector having the GFP marker. To check the transfection after 72 hours, I performed western blot analysis. ...
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DNA Extraction
(1)
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Omega Bio-Tek, Inc.
October 13, 2015
We constantly have to sequence plasmid DNA in our lab. Before going to a maxi prep we always do a mini prep to send it for sequencing and ...
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Good Isotype Control for Flow
(1)
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BioLegend
October 13, 2015
This isotype control was ordered and applied as the isotype control for PE-conjugated flow antibody. It is easy to handle and result was ...
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Kit for cDNA Synthesis from RNA
(1)
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October 13, 2015
RNA isolated from liver tissue by TRIZOL method was used for the synthesis of cDNA by this High-Capacity cDNA Reverse Transcription Kit.
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Antibody Reacts With Mouse IgG2a Strongly
(1)
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Abcam
October 13, 2015
In my test result, Ab97245 reacts with mouse IgG2a heavy chain strongly. Ab97245 reacts with mouse IgG2a light chain slightly, which is the ...
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Standard Protein for Quantifying Mouse TNF-alpha
(1)
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BioLegend
October 13, 2015
We measured the secretion of TNFa in mouse macrophage cells after LPS stimulation in the presence or absence of the fractions/treatment. We ...
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Anti-Ki67 Stains Proliferating Cells in the Mouse Small Intestine
(1)
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Abcam
October 13, 2015
We were interested in examining the proliferation of the intestinal epithelial cells in healthy vs disease states. To do this, we stained ...
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Excellent Antibody for pErk1/2 Immunodetection in Mouse Tissue
(1)
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Cell Signaling Technology
October 13, 2015
Antibody was tested for detection of phospho- p44/42 MAPK (Erk1/2) in mouse tissue protein lysate. Mouse tissue samples were collected in ...
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Good and Easy to Use Antibody
(1)
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BioLegend
October 12, 2015
This antibody was used to detect the CCR2 expression on the human leukemia cell line THP cell line. Cells were stained with this antibody ...
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Western Blot Analysis of Phospho-EGFR (Y1173)
(1)
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Thermo Fisher Scientific
October 12, 2015
This product was used to test the phosphorylation of EGFR after treatment with EGF.
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Sensitive and Quantitative Detection for Mouse Anti-Nuclear Antibodies
(1)
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Alpha Diagnostic International Inc
October 12, 2015
We are interested in detecting anti-nuclear antigen autoantibodies (autoimmune predisposition) in aged mouse serum and comparing that with ...
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Excellent p300 CT Antibody
(1)
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MilliporeSigma
October 12, 2015
In the present study, mammalian cells were tested for p300 prorein levels in response to treatment. Equal amount of proteins were resolved ...
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ETF Maxi Kit
(1)
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QIAGEN - Sample to Insight
October 12, 2015
Using the ETF Maxi kit from Qiagen allows fast and efficient isolation of plasmid DNA suitable for transfection and cloning. In as little ...
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Proliferation Assay for Flow Cytometry Using Edu
(1)
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October 12, 2015
We set out to investigate the proliferative properties of U87 Glioblastoma cell lines. Since we were not convinced using BrdU Assays because...
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Great Antibody!
(1)
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BioLegend
October 12, 2015
This is a great antibody! It is bright, and the recommended dilutions for flow cytometry turned out to be the best after doing titrations. ...
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Easy to Perform, Difficult to Optimize for Good Data
(1)
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Abcam
October 09, 2015
As part of our interest in proteostasis, we have used this kit to measure proteasomal activity. Commercially, there seems to be two ...
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Western Blot Analysis of MTCO2
(1)
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Abcam
October 09, 2015
We used MTCO2 as a marker of mitochondrial fraction in our subcellular fractionation studies.
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ICC/IF Staining of XBP1s-GFP-Transfected Multiple Myeloma Cell Lines
(1)
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BioLegend
October 09, 2015
This antibody was used in our study of proteasome inhibitor resistence in multiple myeloma published in Cancer Cell (Leung-Hagesteijn et al,...
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Extremely Strong Antibody Against GST
(1)
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Santa Cruz Biotechnology, Inc.
October 09, 2015
The aim of this experiment was to study the function of a variety of yeast G1 cyclins by expressing them in budding yeast Saccharomyces ...
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Good APC Conjugate to Stain CSF-1R Myeloid Cells in Mouse
(1)
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BioLegend
October 09, 2015
In vitro cultured bone marrow derived cells were checked for the expression of CSF-1R under osteoclast differentiation conditions from mice.
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Gene Expression Inducer for Bacteriological Assays
(1)
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October 09, 2015
I studied protein-protein interaction using bacteriomatch two and three hybrid system in X-gal indicator plates and used IPTG and Arabinose ...
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Good Antibody
(1)
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October 09, 2015
We routinely use this antibody in our 8-10 color multi parameter flow cytometry analysis and the CD8a antibody conjugated to APC-eF780 works...
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Measurement of Protein Synthesis Through Ribosmal Protein s6
(1)
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Cell Signaling Technology
October 08, 2015
The general aim of this project was to assess protein synthesis in a variety of treatment groups using ribosomal protein s6 (rps6), a ...
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Western Blot Analysis of EGFR
(1)
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Santa Cruz Biotechnology, Inc.
October 08, 2015
This product was used to detect EGFR through western blot analysis in cancer cells.
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Mouse IFN Alpha ELISA Kit for Use on Murine Sera
(1)
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PBL Assay Science
October 08, 2015
Though luminex kits can measure the concentration of multiple cytokines from within a serum sample, mouse IFN alpha is not within those kits...
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Goat Anti-Rabbit AF488 is a Great Secondary Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
October 08, 2015
When a rabbit-derived primary antibody is used to stain an antigen of interest, an anti-rabbit secondary antibody is necessary to visualize ...
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Fast and Excellent Plasma DNA Isolation
(1)
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EpiGentek
October 08, 2015
We plan to identify colon cancer specific genetic mutations from patient plasma samples. DNA isolation was needed to prepare whole genome ...
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Good Antibody for p70 S6 Kinase Immunodetection
(1)
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Cell Signaling Technology
October 08, 2015
The aim of the experiment was to test the effect of growth factor treatment on p70 S6 kinase phosphorylation in mammalian cells. ...
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Anti-Human CD4 Antibody for FACS Analysis
(1)
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October 08, 2015
I use this antibody for the staining of human CD4+ T cells in PBMCs and subsequent FACS analysis. This antibody was of affordable price and ...
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Excellent Antibody for Immunodetection of NOTCH3 in Mammalian Cells
(1)
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Cell Signaling Technology
October 07, 2015
Mammalian cells were cultured up to 70 percent confluence, serum starved for 12h and treated with growth factor. Proteins were extracted in ...
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Bim in Jurkat Cells
(1)
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Cell Signaling Technology
October 07, 2015
The aim of the research was to determine the levels of Bim in Jurkat cells after treatment with various cytokines. Jurkat cells were treated...
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High Fidelity Restriction Enzyme for Cloning
(1)
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New England Biolabs
October 07, 2015
I used this EcoRV restriction enzyme along with BglII for the cloning of my gene of interest into a plasmid vector. Subsequently I used this...
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Immunocytochemistry of EGFR
(1)
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Cell Signaling Technology
October 07, 2015
We were testing this antibody to stain EGFR on cancer cells through immunocytocytochemistry.
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Serum Free Neural Stem Cell Media for Culturing Glioma Cancer Stem Cells
(1)
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Thermo Fisher Scientific
October 07, 2015
This product was used to culture glioma stem cells from U87 cells. The product is a must to add when culturing cancer stem cells.
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Antibody to Study Fumarate Metabolisam
(1)
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Cell Signaling Technology
October 07, 2015
We are studying fumarate metabolism because in some cancers fumarate level is extremely high. We needed to find this fumarase specific band ...
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Western Blot
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Human
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Adipocyte
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Microvascular Endothelial Cell
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HeLa
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