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Antibodies
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Date Published
TCRb PE FACS Antibody
(1)
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BD Biosciences
September 22, 2015
I use this antibody to gate specifically on T cells in order to distinguish between gd T cells and ab T cells in the gut. In addition, I use...
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Extremely Strong Antibody Against Biotin
(1)
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Abcam
September 22, 2015
This antibody detects trace amounts of biotinylated proteins with great ease at the recommended dilution.
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Rhot1 Antibody
(1)
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Novus Biologicals, a Bio-Techne Brand
September 21, 2015
We had previously used other Rhot1 antibodies to determine the levels of Rhot1 in human and mouse lungs. We were not able to get clear bands...
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Excellent Antibody for Detecting Endogenous Ezh2
(1)
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Cell Signaling Technology
September 21, 2015
This Cell Signaling Rabbit Ezh2 antibody detects small amounts of endogenous Ezh2 with great ease at the recommended (1:1000) dilution.
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gd TCR PerCP-eFluor 710 for FACS
(1)
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September 21, 2015
I preferentially use this antibody to define gd T cells in the colon. For this, I firstly isolate the cells of the colon with a common cell ...
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Protease Inhibitor Cocktail
(1)
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Roche
September 21, 2015
Use this protease inhibitor cocktail when making protein lysates for western blotting. Easy to dissolve tablets make it easy to make the ...
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Sensitive But Time-Consuming Protein Assay
(1)
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September 21, 2015
Measuring protein concentration is one of the first steps in our workflow. We use this assay because its 96 well microplate format works ...
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Good Mounting Media
(1)
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Electron Microscopy Sciences
September 21, 2015
This product is used to mount fluorescent specimens onto slides to reduce signal loss. It is a ready to use product with no dilutions or ...
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Nice Kit to Purify PCR Products
(1)
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QIAGEN - Sample to Insight
September 18, 2015
This kit is used to purify PCR products after performing PCR. The kit removes primers as well as proteins and samples run through the kit ...
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Western Blot Analysis of Na+/K+-ATPase Alpha 1
(1)
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Santa Cruz Biotechnology, Inc.
September 18, 2015
We used this product as a plasma membrane marker protein for our subcellular fractionation assays.
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Great Secondary Antibody for Immunofluorescence
(1)
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Thermo Fisher Scientific
September 18, 2015
We study sinus disease in humans, and specifically we were looking at epithelial tight junction staining in ex vivo cultured epithelium.
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Works Poorly in Mouse Cells
(1)
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Abcam
September 18, 2015
I tried this antibody in Western Blot studies using mouse embryonic fibroblast cells and observed that it poorly detects Dnmt1 protein at ...
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Anti-Gamma H2A.X Antibody
(1)
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Abcam
September 18, 2015
We are using human dental fibroblast cells study oxidants. We measure oxidant induced DNA damage by induction of H2AX in cell lysates.
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HDAC2 Antibody for Immunoblotting
(1)
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Abcam
September 17, 2015
We tested different HDAC2 human antibodies for our samples and we found ab32117 antibody works good for human tissue lysate and cell lysates...
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Good Native Sample Buffer
(1)
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Bio-Rad
September 17, 2015
I use this buffer when running a native sample gel i.e. non-denaturing. Sample buffer comes in 2X stock that you just dilute 1:1 with sample...
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Good Kit for Silver Staining
(1)
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Thermo Fisher Scientific
September 17, 2015
This is a good easy to use kit for silver staining protein samples. I have used this kit to detect femtomol concentrations of proteins and ...
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Excellent Antibody to Detect Pak2 in Mammalian Cell Line
(1)
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Cell Signaling Technology
September 17, 2015
Pak2 levels were detected in mammalian cells treated with growth factor. Equal proteins were resolved, western transferred and probed for ...
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Mitotracker Staining Before Fixation
(1)
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Thermo Fisher Scientific
September 17, 2015
The cell-permeant MitoTracker® contain a mildly thiol-reactive chloromethyl moiety and is used in live cells for labeling mitochondria. ...
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Reliable primary antibody in western blot analysis
(1)
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Cell Signaling Technology
September 17, 2015
We carry out subcellular fractionation and verify the isolated fractions by western blot analysis. We have had good experience with Cell ...
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Excellent Intracellular Staining Antibody
(1)
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R&D Systems, a Bio-Techne Brand
September 16, 2015
We were interested in looking at the expression of DAP-12 protein in specific subset of myeloid cells from human PBMCs. DAP-12 monoclonal is...
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Anti-HDAC2 (Phospho S394) Antibody for Mouse
(1)
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Abcam
September 16, 2015
Anti-HDAC2 phospho S394 antibody for mouse lung tissues. Mouse exposed to cigarette smoke shows increased HDAC2 phosphorylation compared to ...
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Good cDNA Kit
(1)
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Quanta BioSciences
September 16, 2015
This is a 1 step cDNA kit that has everything needed for cDNA synthesis in a single mix. This kit is used often to convert RNA to cDNA and I...
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Reliable Hot Plate Stirrer
(1)
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VWR Scientific Products
September 16, 2015
This hot plate stirrer is used to warm up solutions in the lab. It is also used to mix solutions in the lab with a magnetic stir bar. This ...
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Protein A Sephorose CL-4B for Immunopreciptation Application
(1)
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September 16, 2015
We generally used Agarose A beads to pull down a particular protein of interest by immunoprecipitaion.
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Best Glow Luminescence Reagent
(1)
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September 16, 2015
This product is easy to use, has high sensitivity, a long lasting signal and no odor.
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Excellent Phospho-eIF4E (Ser209) Antibody for Immunoblotting
(1)
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Cell Signaling Technology
September 16, 2015
Phospho-eIF4E (Ser209) levels were detected in mammalian cells treated with PDGF for various time points. Selection of antibody was based on...
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Serum Removal - Proteomics of Serum samples
(1)
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SERVA
September 15, 2015
Comparative analysis of normal & infected human patients’ peripheral blood serum proteome yields a potential approach for diagnostic or ...
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Product to Screen the Small Molecule microRNA Inhibitors Using Dual Luciferase System
(1)
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September 15, 2015
We do work on small molecule inhibitors against microRNA which are involved in breast and liver cancer progression. To find small molecule ...
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Bioss Anti-TPSB2/MCPT6 Stains Intestinal Mast Cells
(1)
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Bioss Inc.
September 15, 2015
The goal of the experiment here was to examine whether mast cells would infiltrate the small intestine of a mouse in a diseased state. ...
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Capillary Pistons for Use with a Positive Displacement Pipette in Murine Mucosal Sampling
(1)
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Gilson
September 15, 2015
For mucosal sampling of murine vaginal vaults, for either antibody or cytokine analyses following vaccination for example, an establish ...
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Western Blot Analysis of MFN2
(1)
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Cell Signaling Technology
September 15, 2015
Our laboratory is focused on studying the role of EGFR in de novo fatty acid synthesis and mitochondrial dynamics. In this experiment, we ...
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Bcl-2 in Mouse T cells
(1)
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Cell Signaling Technology
September 15, 2015
The aim of the research was to measure Bcl-2 levels in primary mouse T cells after 24h of treatment by Western blot. For this experiment, we...
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Bad Detection of Phospho-PKD/PKCµ (Ser744/748)
(1)
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Cell Signaling Technology
September 15, 2015
The aim of our study was to test the time course effect of growth factor on Phospho-PKD/PKCµ (Ser744/748) levels in mammalian cell line. For...
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This kit works very well and yields good quality plasmids suitable for transfection and other downstream applications
(1)
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September 14, 2015
I was looking for high quality plasmid DNA for transfection studies and used Qiagen Midikit that worked really well. The quality and yield ...
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Product to Study Whether Protein of Interest is Localized in Cytoplasm or Nucleus
(1)
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September 14, 2015
We are studying our protein of interest's mechanism of action because ideally it is in the cytoplasm but based off of literature we ...
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Excellent p38alpha Antibody
(1)
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Santa Cruz Biotechnology, Inc.
September 14, 2015
Mammalian cells were treated with growth factor for different time points and proteins were extracted. Equal amount of proteins from each ...
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Actin Antibody for Human Samples
(1)
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Santa Cruz Biotechnology, Inc.
September 14, 2015
We had problems in identifying a good Actin antibody that works for both human and mouse samples. Based on our search for actin antibody, we...
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C Tubes & gentleMACS Dissociator for Tumor Dissociation
(1)
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Miltenyi Biotec
September 14, 2015
Miltenyi Biotech's C-Tubes are designed to streamline tissue dissociation, and are very helpful in doing so. By using set programs on the ...
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Positive Displacement Pipette and Tips for Mucosal Sampling
(1)
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Gilson
September 14, 2015
There are multiple techniques for the sampling of the murine vaginal mucosa, including PVA swabs and PBS washing. For minimal invasiveness ...
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Senescence ß-Galactosidase Staining Kit for Cells In Vitro
(1)
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Cell Signaling Technology
September 11, 2015
We used this SA-beta-gal staining kit for determining senescence in cultured mouse bone marrow derived MSCs. We were able to detect SA-beta ...
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Good ECL Solution
(1)
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September 11, 2015
Equal amounts (10 micrograms) of protein was resolved in SDS-PAGE and western transferred onto PVDF membrane. Transferred blot was probed ...
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Good XbaI Restriction Enzyme
(1)
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New England Biolabs
September 11, 2015
This is a good source of the XbaI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning ...
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Good Anti CD74 Antibody for Intracellular FACS
(1)
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BD Biosciences
September 11, 2015
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-...
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Ly6C Antibody
(1)
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BioLegend
September 11, 2015
In this experiment we were examining monocytes in the blood of WT and KO mice.
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GAPDH Antibody That Works in Mice and Humans
(1)
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Santa Cruz Biotechnology, Inc.
September 11, 2015
We tested different antibodies that works for loading control GAPDH and we found sc-365062 works well in our hands for both mouse and human ...
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Overexpression of FoxM1 in Glioblastoma, Breast Cancer and Colorectal Cancer
(1)
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Cell Signaling Technology
September 11, 2015
We are working on FoxM1's role in colorectal cancer and trying to find out the molecular mecanism. This product is user-friendly and allows ...
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Product to Study Stat3 Role in Proteasomal Degradation.
(1)
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Cell Signaling Technology
September 10, 2015
Our study mainly focused on aberrations in proteosomal degradation and how Stat3 is helpful for cell proliferation.
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GFP Antibody That Works Really Well
(1)
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Rockland Immunochemicals, Inc.
September 10, 2015
The purpose of our experiment was to study the expression of cytokine receptor GFP knock-in by immunofluorescence. GFP detection otherwise ...
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Phospho-mTOR Antibody Used for Western blot
(1)
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Cell Signaling Technology
September 10, 2015
For this antibody, follow the protocol, 1:1000 dilution for the primary antibody.
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Good BmtI Restriction Enzyme
(1)
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New England Biolabs
September 10, 2015
This is a good source of the BmtI restriction enzyme. I use this product when doing diagnostic restriction digests or in molecular cloning ...
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Western Blot
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Human
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Adipocyte
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Microvascular Endothelial Cell
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HeLa
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