Review of CD4+ T Cell Isolation Kit via Negative Selection

Results Summary

This isolation kit is very simple to use and the steps can be completed in about 30 mins (very short compared to Miltenyi kits that require the samples be run through a column for isolation). However, the purity of the cells has been quite variable from experiment to experiment. My general observation is that better purity is obtained if you are only isolating cells from one mouse. Once you start to pool multiple animals for a big isolation, the purity drops from ~90% to about 78-82%. And no, I did not go over the max limit of cells suggested by the manufacturer.