Nice DNA Ladder

Harvard Medical School
Medicine
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs

Product Name:

100 bp ladder

Catalog Number:

N0467S

Being a molecular biologist, I often use gel electrophoresis to compare the size of amplicons. Here, I am showing the amplification of Ampicillin resistance marker from the target expressing clones. This ladder works great and is easily available. Based on my experience, I am fond of NEB enzymes and ladders.

Experimental Design and Results Summary

Application

Gel electrophoresis

Starting Material

PCR products and DNA ladder

Protocol Overview

I loaded 5 microlitres of NEB ladder (100 bp) and 20 microlitres of PCR products.

Tips

Thaw the ladder properly before loading.

Results Summary

Most of the clones screened revealed ampicillin resistance gene expression hence there is confirmed transformation.

Additional Notes

None

Related Categories

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Summary

The Good

Cheap, long lasting, and uniform bands

The Bad

None

The Bottom Line

All in all, it is a good ladder

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