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Date Published
Good TGF-b1 Protein (240-B)
(1)
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R&D Systems, a Bio-Techne Brand
January 18, 2023
To induce EMT for BT20 cells, 10 ng/ml TGF-b1 was administered for incubation of 72 h. The cells were collected for fractionation using NE-...
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Great CD3epsilon Antibody for Flow
(1)
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BioLegend
January 18, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Fast and Easy Miniprep Kit
(1)
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New England Biolabs
January 18, 2023
This kit allows quick and easy purification of high quality plasmid DNA, that can be used to transfect cells.
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Good Phospho-S6 Ribosomal Protein mAb for Western Blot
(1)
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Cell Signaling Technology
January 18, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Good Anti-Mouse CD23 BV711
(1)
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BD Biosciences
January 18, 2023
Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against ...
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HLA-A2 Staining
(1)
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BioLegend
January 18, 2023
I use this anti-HLA-A2 antibody to determine the HLA phenotype of human T cells. Fluorescent signal is good, allowing an easy distinction ...
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Reliable and Bright Option to Stain HLA-DR
(1)
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BD Biosciences
January 17, 2023
Our research aims to understand how some CD4 and CD8 T cells become autoreactive in melanoma patients treated with check point inhibitors. ...
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Fixable Viability Dye for Dead Cell Staining
(1)
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January 17, 2023
This viability dye irreversibly labels dead cells. I use it to exclude dead cells from FACS analysis.
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Perfect MAPK Antibody
(1)
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Cell Signaling Technology
January 17, 2023
Band was detected at 40kDA after incubating the membrane overnight at 4 °C. Cells were induced with TGF-b for 48 hours in starvation medium ...
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Super Bright and Economical CD62L Antibody
(1)
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BioLegend
January 17, 2023
I used this antibody to characterize T cell activation and differentiation in C57BL/6 mice. I used this antibody on the murine model of ...
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Versatile PD-1 Antibody Detectable on Most Flow Cytometers
(1)
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BioLegend
January 17, 2023
I wanted to study T cell exhaustion/ regulation in mice during the infection with Plasmodium berghei ANKA, a murine model for malaria. I ...
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CD3 Antibody for T Cell Panels
(1)
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BD Biosciences
January 17, 2023
I used this antibody to identify T cells in C57BL/6 mice in several organs, to further study Tr1 cells and other subsets.
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CD44 Antibody with Little Spillover
(1)
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BD Biosciences
January 17, 2023
I used this antibody to analyze T cell differentiation in a panel also B cells, monocytes, DCs and NK cells in C57BL/6 mice infected with ...
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DNA Gel Extraction with Monarch Buffer
(1)
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New England Biolabs
January 13, 2023
I use this buffer to extract DNA fragment from agarose gel. This buffer allows the dissolution of the agarose slice containing the DNA ...
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Great Ly-6G/Ly-6C Antibody for Flow
(1)
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BioLegend
January 13, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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EGF (236-EG) Protein Works Well
(1)
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R&D Systems, a Bio-Techne Brand
January 13, 2023
To induce EMT for BT20 cells, 20 ng/ml EGF was administered for incubation of 72 h. The cells were collected for fractionation using NE-PER ...
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Anti-RUNX1 Antibody
(1)
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R&D Systems, a Bio-Techne Brand
January 13, 2023
To examine the expression of different RUNX1 fusion proteins caused by intragenic rearrangements, antibodies produced from different ...
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Great CD11b Antibody for Flow
(1)
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BioLegend
January 13, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Generating Chemically Competent E.coli
(1)
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January 13, 2023
This kit is designed for the generation of chemically competent E.coli without heat shocking, but I find it is still necessary to perform a ...
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PD-L1 Antibody is Detectable on Multiple Cell Types
(1)
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Thermo Fisher Scientific
January 12, 2023
I tested this antibody to identify co-inhibitory molecules and their ligands on T cells in a large panel which also analyses B cells, ...
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Cryopreservation with Synth-A-Freeze Medium
(1)
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January 12, 2023
Really great medium to cryopreserve primary human cells lines, such as lymphocytes. Cell viability and recovery after thawing is truly ...
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Great LAG-3 Antibody for the Detection of Tr1 Cells
(1)
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BioLegend
January 12, 2023
I used this antibody to characterize and sort Tr1 cells (identified as CD49b+ LAG-3+ CD4 T cells) in mice infected with Plasmodium berghei ...
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Reliable Antibody for High MW Loading Control
(1)
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Cell Signaling Technology
January 11, 2023
I use this primary antibody to detect the protein vinculin as a loading control for western blots. It is a higher MW than GAPDH so can be a ...
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Ly6C Antibody with Great Resolution and Little Spillover
(1)
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BioLegend
January 11, 2023
I used this antibody to further define and characterize the myeloid compartment of C57BL/6 mice, whilst also staining this marker on T cells...
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Phospho-SAPK/JNK Antibody for Western Blot
(1)
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Cell Signaling Technology
January 11, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Good Option to Stain for CXCR5 in Human (CD185)
(1)
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BD Biosciences
January 11, 2023
Our research aims at understanding how some CD4 T cells become autoreactive in melanoma patients treated with checkpoint inhibitors. CD4 T ...
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Great CD68 Antibody For Flow Cytometry
(1)
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BioLegend
January 11, 2023
Anergy/exhaustion of primary T cells was measured through flow cytometry by analyzing CD68 expression in response to IL-2 stimulation.
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Easy Cloning with NEBuilder HiFi DNA Assembly Master
(1)
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New England Biolabs
January 11, 2023
This mix allows the assembly of several DNA fragments into a vector. It is one of the easiest way to do cloning that I know about, but at a ...
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Great mTOR Antibody for WB
(1)
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Cell Signaling Technology
January 10, 2023
Epithelial cells (A549) were seeded in complete medium (10% F-12k) and starved for 6 hours with 2%FBS. The cells were treated with eiF4E ...
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Bright Ki-67 Antibody That Can Be Used Economically
(1)
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BioLegend
January 10, 2023
I tested this antibody to characterize T cells in a large panel which also analyses B cells, macrophages, monocytes, DCs and NK cells. Ki-67...
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Expensive but Very Useful LAG-3 Antibody in Large Panels
(1)
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BD Biosciences
January 10, 2023
I used this antibody to analyze T cells in C57BL/6 mice infected with Plasmodium berghei ANKA, the murine model for malaria, in different ...
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NK Expansion with IL-2
(1)
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Miltenyi Biotec
January 10, 2023
I use Recombinant human IL-2 Improved Sequence (IS) at 500 UI/mL to expand and activate NK cells. It works very well.
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p21 Waf1/Cip1 (12D1) Rabbit mAb Antibody for Western Blot
(1)
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Cell Signaling Technology
January 10, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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Easy to Use, Affordable CD27 Antibody
(1)
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BioLegend
January 10, 2023
I used this antibody to analyze the maturation from naïve to effector T cells in C57BL/6 in various experiments. Cells were isolated from ...
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Fast and Easy Cloning for Sequencing
(1)
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January 09, 2023
Cloning is really fast and easy with this kit, making sequencing very easy. PCR products amplified with Taq polymerase can be directly ...
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Cloning Reactions with RCutSmart Buffer
(1)
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New England Biolabs
January 09, 2023
I use this buffer to prepare DNA fragments for downstream cloning. Then I perform an agarose gel electrophoresis migration of the enzyme ...
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p70 S6 Kinase Antibody for Western Blot
(1)
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Cell Signaling Technology
January 09, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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MHC Expression with IFN-Gamma
(1)
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January 09, 2023
I use this Recombinant Human IFN-gamma to enhance the expression of surface major histocompatibility complex class I on my cells.
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Fast and Easy DNA Purification with Monarch Kit
(1)
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New England Biolabs
January 09, 2023
I use this kit to purify PCR products and restriction enzyme digestion products. It works very well, giving high quality DNA that can be ...
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Excellent CD3 Pe-Cy7 antibody
(1)
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BioLegend
January 09, 2023
Our lab uses a lot of CD3 for a variety of tests to identify T cells in humans. This antibody from BioLegend is excellent. It was easy to ...
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Plasmid Removal With DPNI
(1)
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New England Biolabs
January 06, 2023
I use this enzyme to remove plasmid template after PCR, before the cloning step. Cloning works very well this way.
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NK Cell Expansion with Gibco Medium
(1)
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January 06, 2023
I use CTS™ NK-Xpander™ Medium supplemented with 5% human AB serum and 500 UI /mL IL-2 to expand NK cells isolated from fresh human PBMC.
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a-CD39 with Exceptional Resolution
(1)
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BioLegend
January 06, 2023
I used this antibody to analyze T cells in a “T cell-co-inhibitor/ exhaustion marker panel” in C57BL/6 mice infected with Plasmodium berghei...
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Good LEF1 Antibody for Flow Cytometry
(1)
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Cell Signaling Technology
January 06, 2023
LEF1 transcription factor expression was analyzed by flow cytometry as an indicator of Wnt signaling activation in endothelial cells.
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Great CD45 Antibody for Flow Cytometry
(1)
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BioLegend
January 06, 2023
Neuroinflammation in a mouse model of viral respiratory infection was measured by analyzing CD45-high cells (leukocytes) and excluding CD45 ...
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Easy NK Cell Isolation with STEMCELL Kit
(1)
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January 06, 2023
I use this kit to isolate NK cells from PBMC. It works with negative selection, thus there are no magnetic particles remaining with the ...
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Good Antibody for Western Blot
(1)
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Cell Signaling Technology
January 05, 2023
I used this antibody diluted in TBST 1:1000 (v/v) and I incubate overnight at 4 °C. The cells (fibroblast) were induced with tgf-b for 24 ...
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CD27 Antibody Works Well in Mice and Humans
(1)
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January 05, 2023
I used this antibody to analyze the differentiation and activation of T cells in a large panel which also analyses B cells, macrophages, ...
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Antibody for Detection of CD155 on Multiple Cell Types
(1)
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BD Biosciences
January 05, 2023
I tested this antibody to identify co-inhibitory molecules and their ligands on T cells in a large panel which also analyses B cells, ...
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Easy PCR with GoTaq G2 Hot Start Green Master Mix
(1)
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Promega
January 05, 2023
PCR are easy to perform with this ready-to-use mix. Moreover, it already contains a gel loading dye, thus you can load your samples on a gel...
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