Generating Chemically Competent E.coli

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Quality of Results

Ease-of-Optimization

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Company:

Zymo Research

Product Name:

Mix & Go! E.coli Transformation Kit and Buffer Set

Catalog Number:

ZT3001

This kit is designed for the generation of chemically competent E.coli without heat shocking, but I find it is still necessary to perform a heat shock.

Experimental Design and Results Summary

Application

Cloning

Starting Material

N/A

Tips

Best results are obtained when lowering the incubation temperature.

Results Summary

I use this kit to generate various chemically competent E.coli cells, Stbl3, Top10 and BL21. It works, but I still need to perform a heat shock for efficient DNA transformation.

DOI or PMID #

N/A

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Summary

The Good

Good quality.

The Bad

Still need heat shocking.

The Bottom Line

Works partially.

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