Anti-RUNX1 Antibody

January 13, 2023

BlueskyReddit
Surgery
University of Pittsburgh
Research Scientist

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Company:

R&D Systems

Product Name:

Anti-RUNX1 antibody

Catalog Number:

AF2399

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: RUNX1 Antibodies

To examine the expression of different RUNX1 fusion proteins caused by intragenic rearrangements, antibodies produced from different immunogens were purchased for Western blotting. The RUNX1 antibody (cat # AF2399, lot # UNC0416031) worked well in the positive control cell line Jurkat but its expression could not be detected in MCF 7 and HCC1937 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates

Primary Incubation

1/1000 dilution 4 degrees overnight

Blocking Agent

5% milk

Secondary Incubation

1:1000 dilution

Tertiary Incubation

Room temperature 1 hour

Detection

ECL

Results Summary

To examine the expression of different RUNX1 fusion proteins caused by intragenic rearrangements, antibodies produced from different immunogens were purchased for Western blotting. The RUNX1 antibody (cat # AF2399, lot # UNC0416031) worked well in the positive control cell line Jurkat but its expression could not be detected in MCF 7 and HCC1937 cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody works.

The Bad

N/A

The Bottom Line

The antibody works.

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