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APC anti-mouse CD45 Antibody
147707
I3/2.3
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Neuroinflammation in a mouse model of viral respiratory infection was measured by analyzing CD45-high cells (leukocytes) and excluding CD45 low cells (microglia).
Flow Cytometry
Mouse brain and spleen tissue
1:200. 30 min on ice
TruStain FcX PLUS (anti-mouse CD16/32) Antibody
N/A
Cytoflex S Flow Cytometer (Beckman Coulter)
Cells expressing high levels of CD45 (leukocytes) from spleen were able to be differentiated from cells expressing mid-to-low levels of CD45 (microglia) in brain using this antibody. As a result, brain-infiltrating leukocytes were identified and quantified by flow cytometry.
Low concentration needed for bright staining.
None.
This antibody works very well to identify and differentiate different CD45 expressing cell types by flow cytometry.